1. bookVolume 67 (2017): Issue 2 (June 2017)
Journal Details
License
Format
Journal
First Published
28 Feb 2007
Publication timeframe
4 times per year
Languages
English
access type Open Access

Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat-soluble vitamins

Published Online: 01 Jun 2017
Page range: 203 - 213
Accepted: 22 Dec 2016
Journal Details
License
Format
Journal
First Published
28 Feb 2007
Publication timeframe
4 times per year
Languages
English
Abstract

HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (Rs > 5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.

Keywords

1. B. Kučerová, L. Krčmová, L. Solichova, J. Plíšek and P. Solich, Comparison of a new high-resolution monolithic column with core-shell and fully porous columns for the analysis of retinol and α-tocopherol in human serum and breast milk by ultra-high-performance liquid chromatography, J. Sep. Sci. 36 (2013) 2223-2230; DOI: 10.1002/jssc.201300242.10.1002/jssc.201300242Search in Google Scholar

2. S. Pous-Torres, J. R. Torres-Lapasió and M. C. García-Álvarez-Coque, Comparison of the performance of Chromolith Performance RP-18e, 1.8μm Zorbax Eclipse XDB-C18 and XTerra MS C18, based on modeling approaches, Anal. Bioanal. Chem. 405 (2013) 2219-2231; DOI: 10.1007/s00216-012-6448-y.10.1007/s00216-012-6448-ySearch in Google Scholar

3. T. Reddy, G. Balammal and A. Kumar, Ultra performance liquid chromatography: an introduction and review, Int. J. Pharm. Res. Anal. 2 (2012) 24-31.Search in Google Scholar

4. J. M. Cunliffe and T. D. Maloney, Fused-core particle technology as an alternative to sub-2-mm particles to achieve high separation efficiency with low backpressure, J. Sep. Sci. 30 (2007) 3104-3109; DOI: 10.1002/jssc.200700260.10.1002/jssc.200700260Search in Google Scholar

5. J. Kirkland, T. Langlois and J. DeStefano, Fused core particles for HPLC columns, Am. Lab. 39 (2007) 18-21.Search in Google Scholar

6. J. J. Salisbury, Fused-core particles: A practical alternative to sub-2 micron particles, J. Chromatogr. Sci. 46 (2008) 883-886; DOI: 10.1093/chromsci/46.10.883.10.1093/chromsci/46.10.883Search in Google Scholar

7. S. Altmaier and K. Cabrera, Structure and performance of silica-based monolithic HPLC columns, J. Sep. Sci. 31 (2008) 2551-2559; DOI: 10.1002/jssc.200800213.10.1002/jssc.200800213Search in Google Scholar

8. S. El Deeb, B. N. Ma and R. Gust, Determination of NiII(3-OMe-salophene) in MCF7 and HT29 cancer cell lines using HR-CS-AAS and in serum albumin using LC with monolithic silica, Microchem. J. 101 (2012) 24-29; DOI: 10.1016/j.microc.2011.09.013.10.1016/j.microc.2011.09.013Search in Google Scholar

9. M. Taha, A. Abed and S. El Deep, Quality Control of Drugs, in Monolithic Silicas in Separation Science Concepts, Syntheses, Characterization, Modelling and Applications (Eds. K. Unger, N. Tanaka and E. Machtejevas), 1st ed., Wiley-VCH, Weinheim-Germany 2011, pp. 189-201.10.1002/9783527633241.ch10Search in Google Scholar

10. S. El Deeb, U. Schepers and H. Wätzig, Evaluation of monolithic C18 HPLC columns for the fast analysis of pilocarpine hydrochloride in the presence of its degradation products, Pharmazie 61 (2006) 751-756.Search in Google Scholar

11. S. El Deeb and H. Wätzig, Performance comparison between monolithic C18 and conventional C18 particle-packed columns in the liquid chromatographic determination of propranolol HCl, Turk. J. Chem, 30 (2006) 543-552.Search in Google Scholar

12. S. El Deeb, L. Preu and H. Wätzig, A strategy to develop fast RP-HPLC methods using monolithic silica columns, J. Sep. Sci. 30 (2007) 1993-2001; DOI: 10.1002/jssc.200700092.10.1002/jssc.200700092Search in Google Scholar

13. S. El Deeb, L. Preu and H. Wätzig, Evaluation of monolithic HPLC columns for various pharmaceutical separations: method transfer from conventional phases and batch to batch repeatability, J. Pharm. Biomed. Anal. 44 (2007) 85-95; DOI: 10.1016/j.jpba.2007.01.045.10.1016/j.jpba.2007.01.045Search in Google Scholar

14. S. El Deeb, U. Schepers and H. Wätzig, Fast HPLC method for the determination of glimepiride, glibenclamide, and related substances using monolithic column and flow program, J. Sep. Sci. 29 (2006) 1571-1577; DOI: 10.1002/jssc.200600056.10.1002/jssc.200600056Search in Google Scholar

15. W. S. Khayoon, B. Saad, B. Salleh, N. A. Ismail, N. A. Abdul Manaf and A. A. Latiff, A reversed phase high performance liquid chromatography method for the determination of fumonisins B1 and B2 in food and feed using monolithic column and positive confirmation by liquid chromatography/tandem mass spectrometry, Anal. Chim. Acta 679 (2010) 91-97; DOI:10.1016/j.aca.2010.09.008.10.1016/j.aca.2010.09.008Search in Google Scholar

16. H. Y. Aboul-Enein, I. Ali and H. Hoenen, Rapid determination of haloperidol and its metabolites in human plasma by HPLC using monolithic silica column and solid-phase extraction, Biomed. Chromatogr. 20 (2006) 760-764; DOI: 10.1002/bmc.593.10.1002/bmc.593Search in Google Scholar

17. M. Hefnawy, M. Al-Omar and S. Julkhuf, Rapid and sensitive simultaneous determination of ezetimibe and simvastatin from their combination drug products by monolithic silica high-performance liquid chromatographic column, J. Pharm. Biomed. Anal. 50 (2009) 527-534; DOI: 1.1016/j.jpba.2009.05.002.10.1016/j.jpba.2009.05.002Search in Google Scholar

18. L. Kaminski, S. El Deeb and H. Wätzig, Repeatability of monolithic HPLC columns while using a flow program, J. Sep. Sci. 31 (2008) 1745-1749; DOI: 10.1002/jssc.200700681.10.1002/jssc.200700681Search in Google Scholar

19. S. Pous-Torres, J. R. Torres-Lapasió, M. J. Ruiz-Angel and M.C. García-Alvarez-Coque, Interpretive optimisation of organic solvent content and flow-rate in the separation of beta-blockers with a Chromolith RP-18e column, J. Sep. Sci. 32 (2009) 2793-2803; DOI: 10.1002/jssc.200900137.10.1002/jssc.200900137Search in Google Scholar

20. K. Cabrera, D. Lubda, H. M. Eggenweiler, H. Minakuchi and K. Nakanishi, A new monolithictype HPLC column for fast separations, J. High Res. Chromatogr. 23 (2000) 93-99; DOI: 10.1002/(SICI)1521-4168(20000101)23.Search in Google Scholar

21. D. Cabooter, K. Broeckhoven, R. Sterken, A. Vanmessen, I. Vandendael, K. Nakanishi, S. Deridder and G. Desmet, Detailed characterization of the kinetic performance of first and second generation silica monolithic columns for reversed-phase chromatography separations, J. Chromatogr. A. 1325 (2014) 72-82; DOI: 10.1016/j.chroma.2013.11.047.10.1016/j.chroma.2013.11.047Search in Google Scholar

22. H. Sklenářová, P. Chocholouš, P. Koblová, L. Zahálka, D. Šatínský, L. Matysová and P. Solich, High-resolution monolithic columns-a new tool for effective and quick separation, Anal. Bioanal. Chem. 405 (2013) 2255-2263; DOI: 10.1007/s00216-012-6561-y.10.1007/s00216-012-6561-ySearch in Google Scholar

23. S. El Deeb, B. N. Ma and R. Gust, Development and validation of a LC method for the separation and determination of the anticancer-active Fe(III) (4-methoxy-salophene) using the new secondgeneration monolith, J. Sep. Sci. 35 (2012) 3434-3438; DOI: 10.1002/jssc.201200734.10.1002/jssc.201200734Search in Google Scholar

24. R. Dabre, N. Azad, A. Schwämmle, M. Lämmerhofer and W. Lindner, Simultaneous separation and analysis of water- and fat-soluble vitamins on multi-modal reversed-phase weak anion exchange material by HPLC-UV, J. Sep. Sci. 34 (2011) 761-772; DOI: 10.1002/jssc.201000793.10.1002/jssc.201000793Search in Google Scholar

25. S. S. Deshpande, Dietary Constituents, in Handbook of Food Toxicology, CRC Press, Boca Raton 2002, pp.184-193.Search in Google Scholar

26. L. Snyder, J. Kirkland and J. Glajch, Completing the Method: Validation and Transfer, in Practical HPLC Method Development, 2nd ed., Wiley, New York 1997, pp. 710-711.10.1002/9781118592014Search in Google Scholar

27. F. Klink, Improving Electrospray LODs by Decreasing Column Diameter, MS solution#2, Separation Science, 2 (2013).Search in Google Scholar

28. K. Croes, A. Steffens, D. Marchand and L. Snyder, Relevance of π-π and dipole-dipole interactions for retention on cyano and phenyl columns in reversed-phase liquid chromatography, J. Chromatogr. A 1098 (2005) 123-130; DOI: 10.1016/j.chroma.2005.08.090.10.1016/j.chroma.2005.08.090Search in Google Scholar

29. Center for Drug Evaluation and Research (CDER), Reviewer Guidance: Validation of Chromatographic Methods, FDA, Washington (1994.Search in Google Scholar

30. B. Buszewski, and S. Noga, Hydrophilic interaction liquid chromatography (HILIC) - a powerful separation technique, Anal. Bioanal. Chem. 402 (2012) 231-247; DOI: 10.1007/s00216-011-5308-5.10.1007/s00216-011-5308-5Search in Google Scholar

31. V. Malik, S. Jain, and P. Malik, HPLC: An analytical technique for pharmaceutical validation of omeprazole, J. Eng. Comp.Appl. Sci. 2 (2013) 1-10.Search in Google Scholar

32. United States Pharmacopeia, USP 30 - NF25, Weight variation of dietary supplement, Rockville (MD) 2007, p. 731.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo