1. bookVolume 71 (2021): Issue 3 (September 2021)
Journal Details
First Published
25 Mar 2014
Publication timeframe
4 times per year
access type Open Access

The Influence of Zinc and Heavy Metals in Feed and Water on the Quality of Cryopreserved Bull Semen

Published Online: 27 Sep 2021
Page range: 326 - 343
Accepted: 10 Aug 2021
Journal Details
First Published
25 Mar 2014
Publication timeframe
4 times per year

This study aimed to assess the influence of different concentrations of zinc (Zn), lead (Pb), mercury (Hg) and cadmium (Cd) found in the feed and water provided to bulls on the concentrations of these metals in cryopreserved bull semen, and to determine their influence onto semen quality parameters. Correlations between heavy metal concentrations in the semen and the quality parameters of semen as estimated by computer-assisted sperm analysis (CASA) and flow cytometry (FC) methods were determined.

A total of 40 cryopreserved semen samples originating from bulls housed in 4 different centers for artificial insemination (A, B, C and D) were examined, making a total of 160 samples. The concentrations of metals and semen quality parameters were determined in cryopreserved semen of 10 bulls from each center, namely 4 samples from each bull. Concentrations of Zn, Pb, Hg and Cd in hay and concentrated feed were within the allowed limits as proposed by the National Research Council (NRC, 2000). A strong negative correlation was detected between curvilinear velocity (VCL) and Zn concentrations in the semen (P < 0.01; r = -0.772) in group D, and a positive correlation of VCL with Pb concentrations (P < 0.05 and r = 0.718) in group B. Mercury concentrations in cryopreserved semen correlated negatively to the percent of live sperm cells with intact acrosomes (V/IA: P < 0.05; r = -0.640) and positively with the percent of dead sperm cells with damaged acrosomes (D/DA: P < 0.01; r = 0.766) in group D. This finding confirms the hypothesis that Hg, even at low concentrations, may cause acrosome damage.


1. Massonyi P, Tomon R, Trandzik J, Nad P, Skalicka M: Concentration of copper, zinc, iron, cadmium, lead and nickel in bull, ram, boar, stallion and fox semen. Trace Elem Electroly 2004, 21:45-49. Search in Google Scholar

2. National Research Council. 2000. Nutrient requirements of beef cattle. 7th Revised Edition, 1996; Update 2000. National Academy Press. Washington, D. C. Search in Google Scholar

3. Asadi MH, Zafari F, Sarveazad A, Abbasi M, Safa M, Koruji M, Yari A, Miran AR: Saffron improves epididymal sperm parameters in rats exposed to cadmium. Nephro Urol Mon 2014, 6:e12125. Search in Google Scholar

4. Babaei H, Abshenas J: Zinc therapy improves adverse effects of long term administration of copper on epididymal sperm quality of rats. Iran J Reprod Med 2013, 11:577-582. Search in Google Scholar

5. Kumar N, Verma RP, Singh LP, Varshney VP, Dass RS: Effect of different levels and sources of zinc supplementation on quantitative and qualitative semen attributes and serum testosterone level in crossbred cattle (Bos indicus x Bos taurus) bulls. Reprod Nutr Dev 2006, 46:663-675. Search in Google Scholar

6. Levaot N, Hershfinkel M: How cellular Zn2+ signaling drives physiological functions. Cell Calcium 2018, 75:53-63. Search in Google Scholar

7. Lee SR: Critical role of zinc as either an antioxidant or a prooxidant in cellular systems. Oxid Med Cell Longev 2018, e9156285:1-11. Search in Google Scholar

8. Foresta C, Garolla A, Cosci I, Menegazzo M, Ferigo M: Role of zinc trafficking in male fertility: from germ to sperm. Hum Reprod 2104, 29:1134–1140. Search in Google Scholar

9. Tan SW, Meiller JC, Mahaffey KR: The endocrine effects of mercury in humans and wildlife. Crit Rev Toxicol 2009, 39:228-269. Search in Google Scholar

10. Boran C, Ozkan U: The effect of zinc therapy on damaged testis in pre-pubertal rats. Pediatr Surg Int 2004, 20:444-448. Search in Google Scholar

11. Björndahl L, Kvist U: A model for the importance of zinc in the dynamics of human sperm chromatin stabilization after ejaculation in relation to sperm DNA vulnerability. Syst Biol Reprod Med 2011, 57:86-92 Search in Google Scholar

12. Tvrda E, Knazicka Z, Lukac N: Selected heavy metals versus antioxidant parameters in bull seminal plasma - a comparative study. J Environ Sci Health A Tox Hazard Subst Environ 2012, 47:1261-1266. Search in Google Scholar

13. Narasimhaiah M, Arunachalam A, Sellappan S, Mayasula VK, Guvvala PR: Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats. Reprod Domest Anim 2018, 53:644-654. Search in Google Scholar

14. Riffo M, Leiva S, Astudillo J: Effect of zinc on human sperm motility and the acrosome reaction. Int J Androl 1992, 15:229-237. Search in Google Scholar

15. Janicki B, Cygan-Szczegieniak D: Zn and Pb concentration in seminal plasma in reference to selected parameters of semiological assessment of bull semen. Folia Biol 2008, 56:97-101. Search in Google Scholar

16. Wang YX, Wang P, Feng W, Liu C, Yang P, Chen YJ, Sun L, Sun Y, Yue J, Gu LJ, Zeng Q, Lu WQ: Relationships between seminal plasma metals/metalloids and semen quality, sperm apoptosis and DNA integrity. Environ Pollut 2017, 224:224-234. Search in Google Scholar

17. ATSDR: Toxicological profile for cadmium. Public Health Service. 1989, ATSDR/TP-8808/08. Search in Google Scholar

18. Tvrda E, Knazicka Z, Lukacova J, Schneidgenova M, Goc Z: The impact of lead and cadmium on selected motility, prooxidant antioxidant parameters of bovine seminal plasma and spermatozoa, J Environ Sci Health A Tox Hazard Subst Environ 2013, 48:1292-1300. Search in Google Scholar

19. Mínguez-Alarcón L, Afeiche MC, Williams PL, Arvizu M, Tanrikut C: Earth Study Team. Hair mercury (Hg) levels, fish consumption and semen parameters among men attending a fertility center. Int J Hyg Environ Health 2018, 22:174-182. Search in Google Scholar

20. Celeghini ECC, de Arruda RP, de Andrade AFC, Nascimento J, Raphael CF: Effects that bovine sperm cryopreservation using two different extenders has on sperm membranes and chromatin. Anim Reprod Sci 2008, 104:119-31. Search in Google Scholar

21. Sariozkan S, Tuncer PB, Bucak MN, Buyukleblebici S, Kinet H: The effect of different egg yolk concentration used with soybean lecithin-based extender on semen quality to freeze bull semen. Eurasia J Vet Sci 2010, 26:45-49. Search in Google Scholar

22. Bucak MN, Tuncer PB, Sariozkan S, Baspinar N, Taspinar M: Effects of antioxidants on post-thawed bovine sperm and oxidative stress parameters: Antioxidants protect DNA integrity against cryodamage. Cryobiology 2010, 61:248-53. Search in Google Scholar

23. Marzec-Wroblewska U, Kaminski P, Lakota P: Influence of chemical elements on mammalian spermatozoa. Folia Biol 2012, 58:7-15. Search in Google Scholar

24. Yamaguchi S, Miura C, Kikuchi K, Cellino FT, Agusa T: Zinc is an essential trace element for spermatogenesis. Proc Natl Acad Sci USA 2009, 26:10859-10864. Search in Google Scholar

25. Mankad M, Sathawara NG, Doshi H, Saiyed HN, Kumar S: Seminal plasma zinc concentration and alpha-glucosidase activity with respect to semen quality. Biol Trace Elem Res 2006, 110:97-106. Search in Google Scholar

26. Hidiroglou M, Knipfel EJ: Zinc in mammalian sperm - a review. J Dairy Sci 1984, 67:1147-1156. Search in Google Scholar

27. Riffo M, Leiva S, Adtudillo J: Effect of zinc on human sperm motility and the acrosome reaction. Int J Androl 1992, 15:229-237. Search in Google Scholar

28. Arabi M: The role of mercury in the etiology of sperm dysfuntion in Holstain bulls. Asian Australas J Anim Sci 2006, 19:335-340. Search in Google Scholar

29. Kushawaha B, Yadav RS, Swain DK, Rai PK, Garg SK: Mercury-induced inhibition of tyrosine phosphorylation of sperm proteins and altered functional dynamics of bull spermatozoa: an in vitro study. Biol Trace Elem Res 2020, 198:478-492. Search in Google Scholar

30. Vince S, Žura Žaja I, Samardžija M, Majić Balić I, Vilić M: Age-related differences of semen quality, seminal plasma, and spermatozoa antioxidative and oxidative stress variables in bulls during cold and warm periods of the year. Animal 2018, 12:559-568. Search in Google Scholar

31. Arabi M: Bull spermatozoa under mercury stress. Reprod Domest Anim 2005, 40:454-459. Search in Google Scholar

32. Martinez CS, Escobar AG, Torres JG, Brum DS, Santos FW: Chronic exposure to low doses of mercury impairs sperm quality and induces oxidative stress in rats. J Toxicol Environ Health 2014, 77:143-154. Search in Google Scholar

33. Gurer H, Ercal N. Can antioxidants be beneficial in the treatment of lead poisoning? Free Radic Biol Med 2000, 10:927-945. Search in Google Scholar

34. Patrick L: Lead toxicity part II: The role of free radical damage and the use of antioxidants in the pathology and treatment of lead toxicity. Altern Med Rev 2006, 11:114-127. Search in Google Scholar

35. Aguiar MGF, Batista BL, Rodrigues LRS, Campiglia AD, Barbosa RM: Determination of trace element in bovine semen samples by inductively coupled plasma mass spectrometry and data mining techniques for identification of bovine class. J Dairy Sci 2012, 12:7066-7073. Search in Google Scholar

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