1. bookVolume 21 (2021): Issue 4 (October 2021)
Journal Details
License
Format
Journal
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
access type Open Access

Attenuative Role of Idebenone on Deltamethrin Mediated Peroxidative Toxicity of Ram Semen Stored at 4°C

Published Online: 28 Oct 2021
Page range: 1377 - 1392
Received: 08 Sep 2020
Accepted: 03 Nov 2020
Journal Details
License
Format
Journal
First Published
25 Nov 2011
Publication timeframe
4 times per year
Languages
English
Abstract

The current study was conducted to evaluate the different concentrations of deltamethrin (DEL; Exp. 1) and the protective role of idebenone (IDB) supplemented with toxic dose of deltamethrin (Exp. 2) during chilled storage of ram semen. Collected samples were pooled and diluted at 500×106 spermatozoa per mL. In Exp. 1, effect of DEL at 1, 2.5, 5, 10, 20, 40, 80 μM levels was evaluated on different variables of spermatozoa motion characteristics. In Exp. 2, different amounts of IDB (2, 4, and 8 μM) concurrent with constant doses of DEL (10 μM) were examined on semen quality upon chilled preservation up to 72 h. Indices of spermatozoa kinematics, functionality of plasma membrane and viability were recorded. Biochemical metabolites were measured in spermatozoa and its medium (extender) at different time points. In Exp. 1, different parameters of spermatozoa kinematics were affected by exposure to DEL in a dose dependent manner. In Exp. 2, combination of IDB with DEL resulted in a significant (P<0.05) increase in total motility, forward progressive motility and curvilinear path velocity compared to DEL group at 24, 48 and 72 h. Simultaneous administration of IDB with DEL increased the percentage of spermatozoa with functional membrane and viability compared to DEL group at 72 h (P<0.05). The amounts of lipid peroxidation index were lower in medium of combination groups compared to DEL group at 48 h and 72 h (P<0.05). Antioxidant capacity was higher in spermatozoa and medium of IDB treated groups compared to DEL group at 72 h (P<0.05). Amounts of total nitrate-nitrite and superoxide dismutase activity of spermatozoa and medium were not affected by treatment (P>0.05). In conclusion, IDB could ameliorate oxidative and peroxidative damages induced by DEL mild toxicity upon cold preservation of ram semen.

Keywords

Abdel Baky N. A., Zaidi Z. F., Fatani A. J., Sayed-Ahmed M. M., Yaqub H. (2010). Nitric oxide pros and cons: The role of L-arginine, a nitric oxide precursor, and idebenone, a coenzyme- Q analogue in ameliorating cerebral hypoxia in rat. Brain Res. Bull., 83: 49–56.Search in Google Scholar

Akalin P. P., Bucak M. N., Güngör Ş., Başpinar N., Çoyan K., Dursun Ş., İli P., Aksoy A., Karaşör Ö. F., Bilgili A., Sariözkan S., Yeni D. (2016). Influence of lycopene and cysteamine on sperm and oxidative stress parameters during liquid storage of ram semen at 5°C. Small Rumin. Res., 137: 117–123.Search in Google Scholar

Andrade A. J. M., Samanta Araújo S., Santana G. M., Ohi M., Dalsenter P. R. (2002). Reproductive effects of deltamethrin on male offspring of rats exposed during pregnancy and lactation. Regul. Toxicol. Pharmacol., 36: 310–317.Search in Google Scholar

Arend N., Wertheimer C., Laubichler P., Wolf A., Kampik A., Kernt M. (2015). Idebenone prevents oxidative stress, cell death and senescence of retinal pigment epithelium cells by stabilizing BAX/Bcl-2ratio. Ophthalmologica, 234: 73–82.Search in Google Scholar

Ben Slima A., Chtourou Y., Barkallah M., Fetoui H., Boudawara T., Gdoura R. (2017). Endocrine disrupting potential and reproductive dysfunction in male mice exposed to deltamethrin. Hum. Exp. Toxicol., 36: 218–226.Search in Google Scholar

Bradford M. M. (1976). A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 7: 248–254.Search in Google Scholar

Bruno V., Battaglia G., Copani A., Sortino M. A., Canonico P. L., Nicoletti F. (1994). Protective action of idebenone against excitotoxic degeneration in cultured cortical neurons. Neurosci. Lett., 178: 193–196.Search in Google Scholar

Bucak M. N., Sariözkan S., Tuncer P. B., Sakin F., Ateşşahin A., Kulaksiz R., Ҫevik M. (2010). The effect of antioxidants on post-thawed Angora goat (Capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Rumin. Res., 8: 24–30.Search in Google Scholar

Buhr M. M., Curtis E. F., Kakuda N. S. (1994). Composition and behavior of head membrane lipids of fresh and cryopreserved boar sperm. Cryobiology, 31: 224–238.Search in Google Scholar

Câmara D. R., Mello-Pinto M. M. C., Pinto L. C., Brasil O. O., Nunes J. F., Guerra M. M. P. (2011). Effects of reduced glutathione and catalase on the kinematics and membrane functionality of sperm during liquid storage of ram semen. Small Rumin. Res., 100: 44–49.Search in Google Scholar

Cardoso S. M., Pereira C., Oliveira C. R. (1998). The protective effect of vitamin E, idebenone and reduced glutathione on free radical mediated injury in rat brain synaptosomes. Biochem. Biophys. Res. Comm., 246: 703–710.Search in Google Scholar

Correa J. R., Zavos P. M. (1994). The hypoosmotic swelling test: its employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane. Theriogenology, 42: 351–360.Search in Google Scholar

Erb M., Hoffmann-Enger B., Deppe H., Soeberdt M., Haefeli R. H., Rummey C., Feurer A., Gueven N. (2012). Features of idebenone and related short-chain quinones that rescue ATP levels under conditions of impaired mitochondrial complex I. PLoS One, 7: e36153.Search in Google Scholar

Eslami M., Jahan-Roshan N., Farrokhi-Ardabili F. (2019). Influence of idebenone on ram semen quality stored at 4°C. Reprod. Domest. Anim., 54: 486–497.Search in Google Scholar

Evans G., Maxwell W. M. C. (1987). Frozen storage of semen. In: Salamon’s artificial insemination of sheep and goats. Butterworths, Wellington, pp. 122–141.Search in Google Scholar

Forshaw P. J., Lister T., Ray D. E. (2000). The role of voltage gated chloride channels in type II pyrethroid insecticide poisoning. Toxicol. Appl. Pharmacol., 163: 1–8.Search in Google Scholar

Ghaniei A., Eslami M., Zadeh Hashem E., Rezapour R., Talebi A. R. (2019). Quercetin attenuates H2O2-induced toxicity of rooster semen during liquid storage at 4°C. J. Anim. Physiol. Anim. Nutr., 103: 713–722.Search in Google Scholar

Green L. C., Wagner D. A., Glogowski J., Skipper P. L., Wishnok J. S., Tannenbaum S. R. (1982). Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal. Biochem., 126: 131–138.Search in Google Scholar

Holt W. V. (2000). Basic aspects of frozen storage of semen. Anim. Reprod. Sci., 62: 3–22.Search in Google Scholar

Holt W. V., North R. D. (1986). Hermotropic phase transitions in the plasma membrane of ram spermatozoa. J. Reprod. Fertil., 78: 447–457.Search in Google Scholar

Imada I., Fujita T., Sugiyama Y., Okamoto K., Kobayashi Y. (1989). Effects of idebenone and related compounds on respiratory activities of brain mitochondria, and on lipid peroxidation of their membranes. Arch. Gerontol. Geriat., 8: 323–341.Search in Google Scholar

Jones R., Mann T. (1977). Toxicity of exogenous fatty acid peroxides towards spermatozoa. J. Reprod. Fertil., 50: 255–260.Search in Google Scholar

Khalatbary A. R., Ahmadvand H., Ghabaee D. N. Z., Malekshah A. K., Navazesh A. (2016). Virgin olive oil ameliorates deltamethrin-induced nephrotoxicity in mice: A biochemical and immunohistochemical assessment. Toxicol. Rep., 3: 584–590.Search in Google Scholar

Koracevic D., Koracevic G., Djordjevic V., Andrejevic S., Cosic V. (2001)). Method for the measurement of antioxidant activity in human fluids. J. Clin. Pathol., 54: 356–361.Search in Google Scholar

Kumar A., Sasmal D., Sharma N. (2014). Deltamethrin induced an apoptogenic signalling pathway in murine thymocytes: exploring the molecular mechanism. J. Appl. Toxicol., 34: 1303–1310.Search in Google Scholar

Kumar A., Sasmal D., Bhaskar A., Mukhopadhyay K., Thakur A., Sharma N. (2016). Deltamethrin-induced oxidative stress and mitochondrial caspase-dependent signaling pathways in murine splenocytes. Environ. Toxicol., 31: 808–819.Search in Google Scholar

Kumar A., Sasmal D., Sharma N. (2018). Mechanism of deltamethrin induced thymic and splenic toxicity in mice and its protection by piperine and curcumin: in vivo study. Drug Chem. Toxicol., 41: 33–41.Search in Google Scholar

Lewis S. E., Boyle P. M., Mc Kinney K. A., Young I. S., Thompson W. (1995). Total antioxidant capacity of seminal plasma is different in fertile and infertile men. Fertil. Steril., 64: 868–870.Search in Google Scholar

Li H. Y., Wu S., Shi N. (2007). Transcription factor Nrf2 activation by deltamethrin in PC12 cells: Involvement of ROS. Toxicol. Lett., 171: 87–98.Search in Google Scholar

Lone F . A., Naikoo M ., Khatun A., Shah R . A., Pampori Z . A., Khan H . M., Ahanger A. A. (2019). Idebenone improves quality of ram sperm by mitigating oxidative stress during cryopreservation. Cryobiology, 90: 15–20.Search in Google Scholar

Marklund S., Marklund G. (1974). Involvement of superoxide anion radical in the auto-oxidation of pyragallol, and a convenient assay for superoxide dismutase. Europ. J. Biochem., 47: 469–474.Search in Google Scholar

Miyamoto M., Coyle J. T. (1990). Idebenone attenuates neuronal degeneration induced by intrastriatal injection of excitotoxins. Exp. Neurol., 108: 38–45.Search in Google Scholar

Miyamoto M., Murphy T. H., Schnaar R. L., Coyle J. T. (1989). Antioxidants protect against glutamate-induced cytotoxicity in a neuronal cell line. J. Pharmacol. Exp. Ther., 250: 1132–1140.Search in Google Scholar

Mordente A., Martorana G. E., Minotti G., Giardina B. (1998). Antioxidant properties of 2,3-dimethoxy-5-methyl-6-(10-hydroxydecyl)-1,4-benzoquinone (idebenone). Chem. Res. Toxicol., 11: 54–63.Search in Google Scholar

Park Y. S., Park J. H., Ko J., Shin I. C., Koh H. C. (2017). mTOR Inhibition by rapamycin protects against deltamethrin-induced apoptosis in PC12 cells. Environ. Toxicol., 32: 109–121.Search in Google Scholar

Pereira C. F., Oliveira C. R. (2000). Oxidative glutamate toxicity involves mitochondrial dysfunction and perturbation of intracellular Ca2+ homeostasis. Neurosci. Res., 37: 227–236.Search in Google Scholar

Romero A., Ramos E., Castellano V., Martínez M. A., Ares I., Martínez M., Martínez-Larrañaga M. R., Anadón A., Anadón A. (2012). Cytotoxicity induced by deltamethrin and its metabolites in SH-SY5Y cells can be differentially prevented by selected antioxidants. Toxicol. In Vitro, 26: 823–830.Search in Google Scholar

Rustin P., von Kleist-Retzow J. C., Chantrel-Groussard K., Sidi D., Munnich A., Rotig A. (1999). Effect of idebenone on cardiomyopathy in Friedreich’s ataxia: a preliminary study. Lancet, 354: 477–479.Search in Google Scholar

Sharma P., Khan I. A., Singh R. (2018). Curcumin and quercetin ameliorated cypermethrin and deltamethrin-induced reproductive system impairment in male wistar rats by upregulating the activity of pituitary-gonadal hormones and steroidogenic enzymes. Int. J. Fertil. Steril., 12: 72–80.Search in Google Scholar

Stern S. T., Potter T. M., Neun B. W. (2010). Hep G2 hepatocyte lipid peroxidation assay. NCL Method GTA-4, Version 1.1.Search in Google Scholar

Wu A., Liu Y. (2000). Apoptotic cell death in rat brain following deltamethrin treatment. Neurosci. Lett., 279: 85–88.Search in Google Scholar

Wu A., Ren T., Hu Q., Liu Y. (2000). Deltamethrin induces altered expression of P53, Bax and Bcl-2 in rat brain. Neurosci. Lett., 284: 29–32.Search in Google Scholar

Yeste M. (2016). Sperm cryopreservation update: Cryodamage, markers, and factors affecting the sperm freezability in pigs. Theriogenology, 85: 47–64.Search in Google Scholar

Zhai H., Cordoba-Diaz M., Wa C., Hui X., Maibach H. I. (2008). Determination of the antioxidative capacity of an antioxidant complex and idebenone: an in vitro rapid and sensitive method. J. Cosmet. Dermatol., 7: 96–100.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo