Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year
access type Open Access

Effect of diet supplementation with Enterococcus durans ED26E/7 and its durancin EntED26e/7 on growth performance, caecal enzymatic activity, jejunal morphology and meat properties of broiler rabbits

Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year

The present study investigates the effects of Enterococcus durans ED26E/7 beneficial strain and its enterocin - durancin EntED26E/7 on selected parameters in rabbits: growth performance, caecal enzymatic activity, jejunal morphometry and meat physico-chemical characteristics. Seventy-two rabbits (aged five weeks, M91 meatline, both sexes) were divided into experimental groups E1 (E. durans ED26E/7 strain; dose 500 μL/animal/day, concentration 109 CFU/mL) and E2 (durancin EntED26E/7; dose 50 µL/animal/day, with activity 12 800 AU/mL) and control group (C). The additives were administered in drinking water for a period of 21 days. All animals remained in good health during the experiment. The highest body weight gain (increase by 1.5% compared to C) was noted in E1 group during ED26E/7 strain application (P<0.001). Both bioactive compounds positively influenced („reduced“) the feed conversion ratio (P<0.001). The ED26E/7 strain and its durancin EntED26E/7 application stimulated the activity of most enzymes tested in the caecum; only the amylolytic and inulolytic activity in E2 group decreased during durancin EntED26E/7 addition. Both additives, but mainl the ED26E/7 strain, showed a tendency to improve the jejunal morhological parameters till the end of the experiment (day 42). The meat physico-chemical parameters were no negatively influenced by the application of E. durans ED26E/7 strain and its durancin EntED26E/7. The diet supplementation with bacteriocinogenic and probiotic E. durans ED26E/7 strain and its durancin EntED26E/7 may improve the growth performance, caecal enzymatic activity and jejunal morphometry of rabbits, without any negative effect on rabbit meat quality.


Amber K.H., Yakout H.M., Hamed Rawya S. (2004). Effect of feedings diets containing Yucca extract or probiotic on growth, digestibility, nitrogen balance and caecal microbial activity of growing New Zealand White rabbits. Proc. 8th World Rabbit Congress, 7-10.09.2004, Puebla, Mexico, pp. 737-745.Search in Google Scholar

AOAC (1995). Official Methods of Analysis of AOAC International. Third ed. Association of Official Analytical Chemists, Washington, DC, USA.Search in Google Scholar

Benato L., Hastie P., O’Shaughnessy P., Murray J.A., Meredith A. (2014). Effects of probiotic Enterococcus faecium and Saccharomyces cerevisiae on the faecal microflora of pet rabbits. J. Small Anim. Pr., 55: 442–446.Search in Google Scholar

Bhatt R.S., Agrawal A.R., Sahoo A. (2017). Effect of probiotic supplementation on growth performance, nutrient utilization and carcass characteristics of growing Chinchilla rabbits. J. Appl. Anim. Res., 45: 304–309.Search in Google Scholar

Burns D.A., Ciurczak E.W. (2007). Handbook of near-infrared analysis (practical Specthoscopy), 3rd edn. CRC Press, 349-369.Search in Google Scholar

Carasi P., Racedo S.M, Jacquot C., El Ie A.M., delos Angeles Serradeli Mm, Urdaci M.C. (2017). Enterococcus durans EP1 a promising anti inflammatory probiotic able to stimulate sIgA and to increase Faecalibacterium prausnitzii abundance. Front. Immunol. 8: 188.Search in Google Scholar

Chiou P.W.S., Yu B., Lin Ch. (1994). Effect of different components of dietary fiber on the intestinal morphology of domestic rabbits. Comp. Biochem. Physiol. (Part A), 108(4): 629-638.Search in Google Scholar

ChrastinováL’., LaukováA., ChrenkováM., PoláčikováM., FormelováZ., KandričákováA., GlatzováE., ŠčerbováJ., Bučko O., Ondruška L’., SzabóováR., Jurčík R. (2018). Effects of enterocin M and durancin ED26E/7 substances applied in drinking water on the selected carcass characteristics and meat quality of broiler rabbits. Arch. Zootech., 21: 5–17.Search in Google Scholar

Dalle Zotte A., (2002). Perception of rabbit meat quality and major factors influencing the rabbit carcass and meat quality. Livest. Prod. Sci., 75: 11–32.Search in Google Scholar

Dalle Zotte A., Ouhayoun J. (1998). Effect of genetic origin, diet and weaning weight on carcass composition, muscle physicochemical and histochemical traits in the rabbit. Meat Sci., 50: 471–478.Search in Google Scholar

Dalle Zotte A., Celia C., SzendrőZs., (2016). Herbs and spices inclusion as feedstuff or additive in growing rabbit diet and as additive in rabbit meat: A review. Livest. Sci., 189: 82–90.Search in Google Scholar

De Vuyst L., Callewaert R., Pot B., 1996. Characterization of the antagonistic activity of Lactobacillus amylovorus DCE471 and large scale isolation of its bacteriocin amylovorin L471. Syst. Appl. Microbiol., 9: 9–20.Search in Google Scholar

EFSA (2019). Panel on Additives and Products or Substances used in Animal Feed (EFSA FEEDAP PANEL). Safety and efficacy of Probiotic Lactina® (Enterococcus faecium NBIMCC8270, Lactobacillus acidophilus NBIMCC 8242, Lactobacillus helveticus NBIMCC 8269, Lactobacillus delrueckei ssp. lactis NBIMCC 8250, Lactobacillus delbrueckei ssp. bulgaricus NBIMCC 8244 and Streptococcus thermophilus NBIMCC 8253) as a feed additive for chickens for fattening and suckling and weaned rabbits. EFSA J., 17(3): 5646.Search in Google Scholar

European Commission (2003). IP/03/1058 Council and Parliament Prohibit Antibiotics as Growth Promoters: Commissioner Byrne Welcomes Adoption of Regulation on Feed Additives; European Commission: Brussels, Belgium.Search in Google Scholar

Falcão–e–Cunha L., Castro–Solla L., Maertens L., Marounek M., Pinheiro V., Freire J., Mourão J.L. (2007). Alternatives to antibiotic growth promoters in rabbit feeding: A review. World Rabbit Sci., 15: 127–140.Search in Google Scholar

Gidenne T., Pérez J.M., Xiccato G., Trocino A., Carabaño R., Villamide M.J., Blas E., Cervera C., Falcao L., Cunha E., Maertens L. (2001). Technical note: Attempts to harmonize chemical analyses of feeds and faeces for rabbit feed evaluation. World Rabbit Sci., 9: 57–64.Search in Google Scholar

Gidenne T., Fortun–Lamothe L. (2002). Feeding strategy for young rabbits around weaning. A review of digestive capacity and nutritional needs. Anim. Sci., 75: 169–184.Search in Google Scholar

Hašek O., PalanskáO. (1976). Determination of water holding capacity in meat by instruments at constant pressure (in Slovak). Poultry Ind., 8: 228–233.Search in Google Scholar

Kalma R.P., Patel V.K., Joshi A., Umatiya R.V., Parmar K.N., Damor S.V., Chauhan H.D., Srivastava A.K., Sharma H.A. (2016). Probiotic supplementation in rabbit: A review. Int. J. Agric. Sci., 8: 2811–2815.Search in Google Scholar

LaukováA., StrompfováV., SkřivanováV., Volek Z., JindřichováE., Marounek M. (2006). Bacteriocin producing strain of Enterococcus faecium EK13 with probiotic character and its application in the digestive tract of rabbits. Biol. Bratisl., 61: 779–782.Search in Google Scholar

LaukováA., ChrastinováĽ., Pogány SimonováM., StrompfováV., PlacháI., ČobanováK., FormelováZ., ChrenkováM., OndruškaĽ.(2012a). Enterococcus faecium AL 41: Its enterocin M and their beneficial use in rabbits husbandry. Probiotics Antimicrob. Proteins, 4: 243–249.Search in Google Scholar

LaukováA., StrompfováV., SzabóováR., KmeťV., Tomáška M. (2012b). Bioactive strains of Enterococcus durans isolated from ewes lump cheese. Slovak Vet. J., 37: 277-278. (in Slovak).Search in Google Scholar

LaukováA., ChrastinováĽ., PlacháI., KandričákováA., SzabóováR., StrompfováV., ChrenkováM., ČobanováK., Žitňan R. (2014). Beneficial effect of lantibiotic nisin in rabbit husbandry. Probiotics Antimicrob. Proteins, 6: 41-46.Search in Google Scholar

Lauková A., Pogány Simonová M., ChrastinováĽ, Kandričáková A., ŠčerbováJ., PlacháI., ČobanováK., FormelováZ., OndruškaĽ., ŠtrkolcováG., StrompfováV. (2017a). Beneficial effect of bacteriocin-producing strain Enterococcus durans ED 26E/7 in model experiment using broiler rabbits. Czech J. Anim. Sci., 62(4): 168–177.Search in Google Scholar

LaukováA., ChrastinováL’., PlacháI., SzabóováR., KandričákováA., Pogány SimonováM., FormelováZ., Ondruška L’., GoldováM., ChrenkováM., StrompfováV. (2017b). Enterocin 55 produced by non rabbit-derived strain Enterococcus faecium EF55 in relation with microbiota and selected parameters in broiler rabbits. Int. J. Agric. Environ. Res., 3: 45–52.Search in Google Scholar

LaukováA., Pogány SimonováM., ChrastinováL’., GancarčíkováS., KandričákováA., PlacháI., ChrenkováM., FormelováZ., Ondruška L’., ŠčerbováJ., StrompfováV. (2018). Assessment of lantibiotic type bacteriocin—Gallidermin application in model experiment with broiler rabbits. Int. J. Anim. Sci., 2: 1028.Search in Google Scholar

LavrenčičA. (2007). The effect of rabbit age on vitro caecal fermentation of starch, pectin,xylan, cellulose, compound feed and its fibre. Animal, 1: 241–248.Search in Google Scholar

Linaje R., Coloma M.D., Pérez–Martínez G., Zúñiga M. (2004). Characterization of faecal enterococci from rabbits for the selection of probiotic strains. J. Appl. Microbiol., 96: 761–771.Search in Google Scholar

MarekováM., LaukováA., Skaugen M., Nes F.I. (2007). Isolation and characterization of a new bacteriocins produced by enviromental isolate Enterococcus faecium AL41. J. Ind. Microbiol. Biotechnol., 34: 533–537.Search in Google Scholar

Marounek M., Vovk S.J., SkřivanováV. (1995). Distribution of activity of hydrolytic enzymes in the digestive tract of rabbits. Br. J. Nutr., 73: 463–469.Search in Google Scholar

MatusevičiušP., Šliaudaryte R., Antoszkiewicz Z., Bednarska A. (2004). A natural way to improve productivity of rabbits using probiotic YEASTURE. Vet. Ir Zootechnica, 26: 61-64.Search in Google Scholar

Matusevičius P., Bartkeviciute Z., Cernauskenie J.;Kozłowski K., Jeroch H. (2011). Effect of probiotic preparation “ToyoCerin (R)” and phytobiotic preparation “Cuxarom Spicemaster” on growing rabbits. Arch. Geflügelkunde, 75(1): 67-71.Search in Google Scholar

Miltko R., Bełżecki G., Kowalik B., SkomiałJ. (2016). Presence of carbohydrate digesting enzymes throughout the digestive tract of sheep. Turkish. J. Vet. Anim. Sci., 40: 271–277.Search in Google Scholar

Oso A.O., Idowu O.M.O., Haastrup A.S., Ajibade A.J., Olowonefa K.O., Aluko A.O., Ogunade I.M., Osho S.O., Bamgbose A.E. (2013). Growth performance, apparent nutrient digestibility, caecal fermentation, ileal morphology and caecal microflora of growing rabbits fed diets containing probiotics and prebiotics. Livest. Sci., 157: 184–190.Search in Google Scholar

Pieniz S., Andreazza R., Anghinoni T., Camargo F., Brandelli A. (2014). Probiotic potential, antimicrobial and antioxidant activities of Enterococcus durans strain LAB18s. Food Control, 37: 251-256.Search in Google Scholar

Pogány SimonováM., LaukováA., ChrastinováL’., StrompfováV., FaixŠ., VasilkováZ., Ondruška L’., Jurčík R., Rafay J. (2009). Enterococcus faecium CCM7420, bacteriocin PPB CCM7420 and their effect in the digestive tract of rabbits. Czech J. Anim. Sci., 54: 376–386.Search in Google Scholar

Pogány SimonováM., LaukováA., Žitňan R., ChrastinováL’. (2015). Effect of rabbit origin enterocin-producing strain Enterococcus faecium CCM7420 application on growth performance and gut morphometry in rabbits. Czech J. Anim. Sci., 60: 509–512.Search in Google Scholar

Pogány SimonováM., ChrastinováL’., LaukováA. (2016). Dietary supplementation of a bacteriocinogenic and probiotic strain of Enterococcus faecium CCM7420 and its effect on the mineral content and quality of Musculus longissimus dorsi in rabbits. Anim. Prod. Sci., 56(Suppl. 12): 2140–2145.Search in Google Scholar

Pogány SimonováM., ChrastinováL’., LaukováA. (2019). Lantibiotic nisin applied in broiler rabbits and its effect on the growth performance and carcass quality. Probiotics Antimicrob. Proteins, 11: 1414–1417.Search in Google Scholar

Pogány SimonováM., ChrastinováĽ., LaukováA. (2020a). Autochtonous strain Enterococcus faecium EF2019(CCM7420), its bacteriocin and their beneficial effects in broiler rabbits—A Review. Animals, 10: 1188.Search in Google Scholar

Pogány SimonováM., ChrastinováĽ., KandričákováA., GancarčíkováS., Bino E., PlacháI., ŠčerbováJ., StrompfováV., Žitňan R., Lauková A. (2020b). Can enterocin M in combination with sage extract have beneficial effect on microbiota, blood biochemistry, phagocytic activity and jejunal morphometry in broiler rabbits? Animals, 10: 115.Search in Google Scholar

Pogány SimonováM., ChrastinováL’., ChrenkováM., FormelováZ., KandričákováA., Bino E., LaukováA. (2020c). Benefits of enterocin M and sage combination on the physico-chemical traits, fatty acid, amino acid, and mineral content of rabbit meat. Probiotics Antimicrob. Proteins, 12: 1235-1245.Search in Google Scholar

Pogány SimonováM., ChrastinováL’., KandričákováA., KubašováI., FormelováZ., ChrenkováM., Miltko R., Belzecki G., StrompfováV., LaukováA. (2020d). Enterocin M and sage supplementation in post-weaning rabbits: Effects on growth performance, caecal microbiota, fermentation and enzymatic activity. Probiotics Antimicrob. Proteins, 12: 732–739.Search in Google Scholar

Pogány SimonováM., ChrastinováĽ., LaukováA. (2020e). Effect of beneficial strain Enterococcus faecium EF9a isolated from Pannon White rabbit on growth performance and meat quality of rabbits. Ital. J. Anim. Sci., 19(1): 650-655.Search in Google Scholar

Polak T., Gašperlin L., Rajar A., Žlender B. (2006). Influence of genotype lines, age at slaughter and sexes on the composition of rabbit meat. Food Technol. Biotechnol., 44: 65–73.Search in Google Scholar

SimonováM., LaukováA. (2004). Isolation of faecal Enterococcus faecium strains from rabbits and their sensitivity to antibiotics and ability to bacteriocin production. Bull. Vet. Inst. Pulawy, 48: 383–386.Search in Google Scholar

Strmiska F, Holčíkova K, SimonováE, Mrázova E, HodekováJ, VotaššakováA, PristašováM, Strmiska J, StrmiskováG, KrupařováM, Gola J, PappajováH, MarešJ, KostkanováE, Ehrenhaft B. (1988). Požívatinové tabuľky I – Potravinárske suroviny, food tables I – primary foods. (in Slovak with English summary and register). Výskumný ústav potravinársky (food research institute), Bratislava, p 189.Search in Google Scholar

SzabóováR., ChrastinováL’., LaukováA., HaviarováM., SimonováM., StrompfováV., FaixŠ., VasilkováZ., ChrenkováM., PlacháI., Mojto J., Rafay J. (2008). Bacteriocin - producing strain Enterococcus faecium CCM4231 and its use in rabbits. Int. J. Prob. Preb., 3: 77–82.Search in Google Scholar

SzabóováR., LaukováA., ChrastinováL’., Pogány SimonováM., StrompfováV., PlacháI., ČobanováK., VasilkováZ., ChrenkováM. (2011). Enterocin 4231 produced by Enterococcus faecium CCM 4231 and its use in rabbits. Acta Vet. Brno, 61(5-6): 523-529.Search in Google Scholar

Vanden Bogaard A.E., Stobberingh E.E. (1999). Antibiotic usage in animals: impact on bacterial resistance and public health. Drugs, 58: 589–607.Search in Google Scholar

Van Soest J.P., Robertson J.B., Lewis B.A. (1991). Methods for dietary fibre, neutral detergent fibre and non starch polysaccharides in relation to animal nutrition. J. Dairy Sci., 74: 3583–3594.Search in Google Scholar

Wiseman J., Villamide M.J., De Blas C., Carabano M.J., Carabano R.M. (1992). Prediction of the digestible energy and digestibility of gross energy of feed for rabbits. 1. Individual classes of feeds. Anim. Feed Sci. Technol., 39: 27–38.Search in Google Scholar

Žitňan R., Voight J., Kuhla S., Wegner J., Chudy A., Schönhausen U., Brna M., Zupcanova M., Hagemeister H. (2008). Morphology of small intestinal mucosa and intestinal weight change with metabolic type of cattle. Vet. Med., 53: 525–532.Search in Google Scholar

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