Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year
access type Open Access

Association between Natriuretic peptide receptor 2 (npr2) rs208158047 polymorphism and fattening performance of young bulls

Published Online: 12 May 2021
Page range: -
Received: 13 Nov 2020
Accepted: 15 Apr 2021
Journal Details
First Published
25 Nov 2011
Publication timeframe
4 times per year

The objective of this study was to determine fattening performance data for Charolais, Limousin and Blonde d’Aquitaine beef cattle and associate these data with NPR2 gene 8:g.59961937 T>C (rs208158047) mutation. Experiments were conducted with 176 beef cattle (77 Charolais, 66 Limousin and 33 Blonde d’Aquitaine) at nine months of age. Experiments lasted for 9 months and animals were slaughtered at the age of 18 months. Cattle body weights were determined at four different periods: beginning of fattening (d0), 60th day of fattening (d60), 120th day of fattening (d120) and at the end of fattening (sw). In terms of rs208158047 mutation of Charolais, Limousin and Blonde d’Aquitaine breeds, TT and CT genotypes were identified, and CC genotype was not encountered. The association of average daily gain (ADG) in d0-d60, d0-d120 and d0-sw periods with the genotypes of rs208158047 mutation was found to be significant (P<0.05). Greater ADGs were observed in rs208158047-CT genotypes compared to rs208158047-TT genotypes. These results indicate that the selection of bovine NPR2 gene could be used to ensure the breeding direction for growth related traits of the beef cattle.


Ahbara A., Bahbahani H., Almathen F., Al Abri M., Agoub M.O., Abeba A., Kebede A, Musa H.H., Mastrangelo S., Pilla F., Ciani E., Hanotte O., Mwacharo J.M. (2019). Genome-Wide variation, candidate regions and genes associated with fat deposition and tail morphology in Ethiopian indigenous sheep. Front. Genet., 9: 699.Search in Google Scholar

Almeida O.A.C., Moreira G.C.M., Rezende F.M., Boschiero C., De Oliveira Peixoto J., Ibelli A.M.G., Ledur M.C., de Novais F.J., Coutinho L.L. (2019). Identification of selection signatures involved in performance traits in a paternal broiler line. BMC Genomics, 20: 449.Search in Google Scholar

Anand-Srivastava M.B. (2005). Natriuretic peptide receptor-C signaling and regulation. Peptides, 26: 1044–1059.Search in Google Scholar

Andersson L. (2012). How selective sweeps in domestic animals provide new insight into biological mechanisms. J. Intern. Med., 271: 1–14.Search in Google Scholar

Bartels C.F., Bükülmez H., Padayatti P., Rhee D.K., van Ravenswaaij-Arts C., Pauli R.M., Mundlos S., Chitayat D., Shih L.Y., Al-Gazali L.I., Kant S., Cole T., Morton J., Cormier-Daire V., Faivre L., Lees M., Kirk J., Mortier G.R., Leroy J., Zabel B., Kim C.A., Crow Y., Braverman N.E., van den Akker F., Warman M.L. Mutations in the transmembrane natriuretic peptide receptor NPR-B impair skeletal growth and cause acromesomelic dysplasia, type Maroteaux. Am. J. Hum. Genet., 75: 27–34.Search in Google Scholar

Blaha M., Nemcova L., Prochazka R. (2015). Cyclic guanosine monophosphate does not inhibit gonadotropin-induced activation of mitogen-activated protein kinase 3/1 in pig cumulus-oocyte complexes. Reprod. Biol. Endocrinol., 13: 1.Search in Google Scholar

Bouwman A.C., Daetwyler H.D., Chamberlain A.J., Ponce C.H., Sargolzaei M., Schenkel F.S., Sahana G., Govignon-Gion A., Boitard S., Dolezal M., Pausch H., Brøndum R.F., Bowman P.J., Thomsen B., Guldbrandtsen B., Lund M.S., Servin B., Garrick D.J., Reecy J., Vilkki J., Bagnato A., Wang M., Hoff J.L., Schnabel R.D., Taylor J.F., Vinkhuyzen A.A.E., Panitz F., Bendixen C., Holm L.E., Gredler B., Hozé C., Boussaha M., Sanchez M.P., Rocha D., Capitan A., Tribout T., Barbat A., Croiseau P., Drögemüller C., Jagannathan V., Vander Jagt C., Crowley J.J., Bieber A., Purfield D.C., Berry D.P., Emmerling R., Götz K.U., Frischknecht M., Russ I., Sölkner J., Van Tassell C.P., Fries R., Stothard P., Veerkamp R.F., Boichard D., Goddard M.E., Hayes B.J. (2018). Meta-analysis of genome-wide association studies for cattle stature identifies common genes that regulate body size in mammals. Nat. Genet., 50: 362–367.Search in Google Scholar

Crispim A.C., Kelly M.J., Guimarães S.E.F., E Silva F.F., Fortes M.R.S., Wenceslau R.R., Moore S. (2015). Multi-trait GWAS and new candidate genes annotation for growth curve parameters in brahman cattle. PloS ONE, 10: e0139906.Search in Google Scholar

de Simoni Gouveia J.J., da Silva M.V.G.B., Paiva S.R., de Oliveira S.M.P. (2014). Identification of selection signatures in livestock species. Genet. Mol. Biol., 37: 330-42.Search in Google Scholar

FAO (2019). World Food and Agriculture – Statistical pocketbook. Rome.Search in Google Scholar

Keller S.R., Taylor D.R. (2008). History, chance and adaptation during biological invasion: Separating stochastic phenotypic evolution from response to selection. Ecol. Lett., 11: 852–866.Search in Google Scholar

Khan R., Raza S.H.A., Guo H., Xiaoyu W., Sen W., Suhail S.M., Rahman A., Ullah I., Abd El-Aziz A.H., Manzari Z., Alshawi, A. (2020). Genetic variants in the TORC2 gene promoter and their association with body measurement and carcass quality traits in Qinchuan cattle. PloS ONE, 15: e0227254.Search in Google Scholar

Kijas J.W., Lenstra J.A., Hayes B., Boitard S., Porto Neto L.R., San Cristobal M., Servin B., McCulloch R., Whan V., Gietzen K., Paiva S., Barendse W., Ciani E., Raadsma H., McEwan J., Dalrymple B., International Sheep Genomics Consortium Members. (2012). Genome-Wide analysis of the world’s sheep breeds reveals high levels of historic mixture and strong recent selection. PloS Biol., 10: e1001258.Search in Google Scholar

Liu G.Y., Raza S.H.A., Zhou L., Abd El-Aziz A.H., Sabek A., Shoorei H., Amjadi M., Gui, L.S. (2020). The genetic polymorphisms of melanocortin-4 receptor gene are associated with carcass quality traits in a Chinese indigenous beef cattle breed. Res. Vet. Sci., 132: 202–206.Search in Google Scholar

Manzari Z., Mehrabani-Yeganeh H., Nejati-Javaremi A., Moradi M.H., Gholizadeh M. (2019). Detecting selection signatures in three Iranian sheep breeds. Anim. Genet., 50: 298–302.Search in Google Scholar

Mastrangelo S., Bahbahani H., Moioli B., Ahbara A., Al Abri M., Almathen F., da Silva A., Belabdi I., Portolano B., Mwacharo J.M., Hanotte O., Pilla F., Ciani E. (2019). Novel and known signals of selection for fat deposition in domestic sheep breeds from Africa and Eurasia. PloS ONE, 14: e0209632.Search in Google Scholar

Michenet A., Barbat M., Saintilan R., Venot E., Phocas F. (2016). Detection of quantitative trait loci for maternal traits using high-density genotypes of Blonde d’Aquitaine beef cattle. BMC Genet., 17: 88.Search in Google Scholar

Moradi M.H., Nejati-Javaremi A., Moradi-Shahrbabak M., Dodds K.G., McEwan J.C. (2012). Genomic scan of selective sweeps in thin and fat tail sheep breeds for identifying of candidate regions associated with fat deposition. BMC Genet., 13: 10.Search in Google Scholar

Nei M., Roychoudhury A.K. (1974). Sampling variances of heterozygosity and genetic distance. Genetics, 76: 379–390.Search in Google Scholar

Olney R.C. (2006). C-type natriuretic peptide in growth: A new paradigm. Growth Horm. IGF Res., 16: S6–14.Search in Google Scholar

Pitt D, Sevane N, Nicolazzi E.L., MacHugh D.E., Park S.D.E., Colli L., Martinez R., Bruford M.W., Orozco-terWengel P. (2019). Domestication of cattle: Two or three events? Evol. Appl., 12: 123–136.Search in Google Scholar

Potter L.R., Abbey-Hosch S., Dickey D.M. (2006). Natriuretic peptides, their receptors, and cyclic guanosine monophosphate-dependent signaling functions. Endocr. Rev., 27: 47–72.Search in Google Scholar

Randhawa I.A.S., Khatkar M.S., Thomson P.C., Raadsma H.W. (2016). A meta-assembly of selection signatures in cattle. PLoS ONE, 11: e0153013.Search in Google Scholar

Raza S.H.A., Khan R., Abdelnour S.A., El-Hack A., Mohamed E., Khafaga A.F., Taha A., Ohran H., Mei C., Schreurs N.M., Zan L. (2019). Advances of molecular markers and their application for body variables and carcass traits in Qinchuan cattle. Genes, 10: 717.Search in Google Scholar

Raza S.H.A., Khan R., Gui L., Schreurs N.M., Wang X., Mei C., Yang X., Gong C. Zan L. (2020c). Bioinformatics analysis and genetic polymorphisms in genomic region of the bovine SH2B2 gene and their associations with molecular breeding for body size traits in Qinchuan beef cattle. Biosci. Rep., 40: BSR20192113.Search in Google Scholar

Raza S.H.A., Khan S., Amjadi M., Abdelnour S.A., Ohran H., Alanazi K.M., Abd El-Hack M.E., Taha A.E., Khan R., Gong C. and Schreurs N.M. (2020b). Genome-wide association studies reveal novel loci associated with carcass and body measures in beef cattle. Arch. Biochem. Biophys., 694: 108543.Search in Google Scholar

Raza S.H.A., Liu G.Y., Zhou L., Gui L.S., Khan R., Jinmeng Y., Chugang M., Schreurs, N.M., Ji, R., Zan L. (2020a). Detection of polymorphisms in the bovine leptin receptor gene affects fat deposition in two Chinese beef cattle breeds. Gene, 758: 144957.Search in Google Scholar

Raza S.H.A., Shijun L., Khan R., Schreurs N.M., Manzari Z., Abd El-Aziz A.H., Ullah I., Kaster N., Shah M.A., Zan L. (2020d). Polymorphism of the PLIN1 gene and its association with body measures and ultrasound carcass traits in Qinchuan beef cattle. Genome, 63: 483-492.Search in Google Scholar

Snelling W.M., Allan M.F., Keele J.W., Kuehn L.A., McDaneld T., Smith T.P.L., Sonstegard T.S., Thallman R.M., Bennett G.L. (2010). Genome-wide association study of growth in crossbred beef cattle. J. Anim. Sci., 88: 837–848.Search in Google Scholar

Sun W., Liu C., Feng Y., Zhuo G., Zhou W., Fei X., Zhang Z. (2017). Macrophage colony-stimulating factor (M-CSF) is an intermediate in the process of luteinizing hormone-induced decrease in natriuretic peptide receptor 2 (NPR2) and resumption of oocyte meiosis. J. Ovarian Res., 10: 68.Search in Google Scholar

Tamura N., Doolittle L.K., Hammer R.E., Shelton J.M., Richardson J.A, Garbers D.L. (2004). Critical roles of the guanylyl cyclase B receptor in endochondral ossification and development of female reproductive organs. Proc. Natl. Acad. Sci., 101: 17300–17305.Search in Google Scholar

Tamura N., Garbers D.L. (2003). Regulation of the guanylyl cyclase-B receptor by alternative splicing. J. Biol. Chem., 278: 48880–48889.Search in Google Scholar

Tsuji T., Kunieda T. (2005). A loss-of-function mutation in Natriuretic peptide receptor 2 (NPR2) gene is responsible for disproportionate dwarfism in cn/cn mouse. J. Biol. Chem., 280: 14288–14292.Search in Google Scholar

Vasques G.A., Arnhold I.J.P., Jorge A.A.L. (2014). Role of the natriuretic peptide system in normal growth and growth disorders. Horm. Res. Paediatr., 82: 222–229.Search in Google Scholar

Wang L., Raza S.H.A., Gui L., Li, S., Liu X., Yang X., Wang S., Zan, L., Zhao C. (2020). Associations between UASMS2 polymorphism in leptin gene and growth, carcass and meat quality traits of cattle: a meta-analysis. Anim. Biotech., doi: 10.1080/10495398.2020.1805327.Search in Google Scholar

Wang S.R., Jacobsen C.M., Carmichael H., Edmund A.B., Robinson J.W., Olney R.C., Miller T.C., Moon J.E., Mericq V., Potter L.R., Warman M.L., Hirschhorn J.N., Dauber A. (2015). Heterozygous mutations in natriuretic peptide receptor-B (NPR2) gene as a cause of short stature. Hum. Mutat., 36: 474–481.Search in Google Scholar

Wei C., Wang H., Liu G., Wu M., Cao J., Liu Z., Liu R., Zhao F., Zhang L., Lu J., Du L. (2015). Genome-wide analysis reveals population structure and selection in Chinese indigenous sheep breeds. BMC Genomics, 16: 194.Search in Google Scholar

Xu L., Bickhart D.M., Cole J.B., Schroeder S.G., Song J., Van Tassell C.P., Sonstegard T.S., Liu G.E. (2015). Genomic signatures reveal new evidences for selection of important traits in domestic cattle. Mol. Biol. Evol., 32: 711–725.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo