1. bookVolume 57 (2013): Issue 3 (September 2013)
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17 Jan 2013
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4 times per year
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access type Open Access

Expression of p16 (INK4a), cytokeratin 19, and Ki-67 in canine laryngeal squamous cell carcinoma

Journal Details
License
Format
Journal
First Published
17 Jan 2013
Publication timeframe
4 times per year
Languages
English

The study aimed at morphological and immunohistochemical characteristics of laryngeal squamous cell carcinomas and their metastases in canine lymph nodes and lungs. Tissue sections were stained using classical technique with haematoxylin and eosin. In addition, immunohistochemical studies were performed with p16, cytokeratin, and Ki-67 antibodies. An expression of all examined antigens was detected in laryngeal tumours, while in tumour metastases only expression of p16 protein and cytokeratin was demonstrated. The results pointed to higher proliferative potential of the primary tumour than of their metastases.

Keywords

1. Agoff S.N., Lin P., Morihara J., Mao C., Kiviat N.B., Koutsky L.A.: p16INK4a expression correlates with degree of cervical neoplasia: A comparison with Ki-67 expression and detection of high-risk HPV types. Mod Pathol 2003, 16, 665-673.Search in Google Scholar

2. Barnard N.J., Hall P.A., Lemoine N.R., Kadar N.: Proliferative index in breast carcinoma determined in situ by Ki67 immunostaining and its relationship to clinical and pathological variables. J Pathol 1987, 152, 287-295.Search in Google Scholar

3. Beaumont P.R., O’Brien J.B., Allen H.L., Tucker J.A.: Mast cell sarcoma of the larynx in a dog: a case report. J Small Anim Pract 1979, 20, 19-25.Search in Google Scholar

4. Brodey R.S., O’Brien J., Berg P., Roszel J.F.: Osteosarcoma of the upper airway in the dog. J Am Vet Med Assoc 1969, 155, 1460-1464.Search in Google Scholar

5. Carlisle C.H., Biery D.N., Thrall D.E.: Tracheal and laryngeal tumors in the dog and cat: Literature review and 13 addictional patients. Vet Radiol 1991, 32, 229-265.Search in Google Scholar

6. Chandrashekaraiah G.B., Rao S., Munivenkatappa B.S., Mathur K.Y.: Canine squamous cell carcinoma: a review of 17 cases. Braz J Vet Pathol 2011, 4, 79-86.Search in Google Scholar

7. Coggins C.R.E.: A review of chronic inhalation studies with mainstream cigarette smoke, in hamsters, dogs, and nonhuman primates. Toxicol Pathol 2001, 29, 550-557.Search in Google Scholar

8. Cooper D., Schermer A., Sun T.T.: Classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations. Lab Invest 1985, 52, 243-256.Search in Google Scholar

9. Dungworth D.L., Hauser B., Hahn F.F., Wilson D.W., Haenichen T., Harkema J.R.: Histological classification of tumors of the respiratory system of domestic animals. WHO, Second Series 1999, 6, 23-24.Search in Google Scholar

10. Eshkoor S.A., Ismail1 P., Rahman S.A., Mirinargesil M., Oshkour S.A.: Increased protein expression of p16 and cyclin D1 in squamous cell carcinoma tissues. BioSci Trends 2009, 3, 105-109.Search in Google Scholar

11. Galus R., Włodarski K.: Importance of the cytokeratins in cancer diagnosis. Pol Merk Lek 2007, 135, 209-211.Search in Google Scholar

12. Gerdes J., Lelle R.J., Pickartz H., Heidenreich W., Schwarting R., Kurtsiefer L., Stauch G., Stein H.: Growth fractions in breast cancers determined in situ with monoclonal antibody Ki-67. J Clin Pathol 1986, 39, 977-980.Search in Google Scholar

13. Giziński S., Boryczko Z., Katkiewicz M., Bostedt H.: Ki-67 protein as a prognostic factor in mammary gland tumors in female dogs. Med Weter 2003, 59, 888-891.Search in Google Scholar

14. Hargis A.M., Thomassen R.W., Phemister R.D.: Chronic dermatosis and cutaneous squamous cell carcinoma in the Beagle dog. Vet Pathol 1977, 14, 218-228.Search in Google Scholar

15. Iwaya K., Mukai K.: Accumulation of ubiquitin-conjugated cytokeratin fragments in tumor cells. Semin Cancer Biol 2005, 15, 309-318.Search in Google Scholar

16. Jones D.L.: Squamous cell carcinoma of the larynx and pharynx in horses. Cornell Vet 1994, 84, 15-24.Search in Google Scholar

17. Kaufmann O., Fietze E., Mengs J., Dietel M.: Value of p63 and cytokeratin 5/6 as immunohistochemical markers for the differential diagnosis of poorly differentiated and undifferentiated carcinomas. Am J Clin Pathol 2001, 116, 823-830.Search in Google Scholar

18. Koenig A., Bianco S., Fosmire S., Wojcieszyn J., Modiano J.: Expression and significance of p53, rb, p21/waf-1, p16/ink-4a, and PTEN tumor suppressors in canine melanoma. Vet Pathol 2002, 39, 458-472.Search in Google Scholar

19. Kozaki M., Nakamura Y., Iguchi M., Kano R., Watanabe S., Fujiwara K., Hasegawa A.: Immunohistochemical analysis of cytokeratin expression in dog skin. J Vet Med Sci 2001, 63, 1-4.Search in Google Scholar

20. Kreipe H., Heidebrecht H.J., Hansen S., Rohlk W., Kubbies M., Wacker H.H., Tiemann M., Radzun H.J., Parwaresch R.: A new proliferation-associated nuclear antigen detectable in paraffin- embedded tissues by the monoclonal antibody Ki-S1. Am J Pathol 1993, 142, 3-9.Search in Google Scholar

21. Lascelles B.D.X., Parry A.T., Stidworthy M.F., Dobson J.M., White R.A.S.: Squamous cell carcinoma of the nasal planum in 17 dogs. Vet Rec 2000, 147, 473-476.Search in Google Scholar

22. Meuten D.J.: Tumors in domestic animals. Iowa State Press, 2002, pp. 51-52, 377-378.Search in Google Scholar

23. Moll R., Franke W.W., Schiller D.L., Geiger B., Krepler R.: The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24.Search in Google Scholar

24. Morris J., Dobson J.: Small Animal Oncology, edited by Blackwell Science, Iowa, 2001, pp. 144-147.Search in Google Scholar

25. Myung N., Kim M., Chung I., Kim H., Jang J.: Loss of p16 and p27 is associated with progression of human gastric cancer. Cancer Lett 2000, 153, 129-136.Search in Google Scholar

26. Nazifi S., Tamadon A., Meimandi Parizi A.H., Mahjoor A., Bigham A.: Squamous cell carcinoma of the larynx in a Pekingese dog. Comp Clin Pathol 2006, 15, 199-202.Search in Google Scholar

27. Nowak M., Madej J.A., Dzięgiel P.: Correlation between the expression of mcm-3 protein and ki-67 antigen in mammary adenocarcinomas of dogs (short communication). Bull Vet Inst Pulawy 2008, 52, 701-705.Search in Google Scholar

28. Nowak M., Madej J.A., Dzięgiel P.: Expression of metallothionein and its correlation with Ki-67 antigen in bitch’s mammary gland adenocarcinomas. Med Weter 2006, 62, 427-431.Search in Google Scholar

29. Nowak M., Madej J.A., Dzięgiel P.: Extent of metallothionein expression in correlation with expression of Ki-67 antigen in soft tissue fibrosarcoma in dogs. Bull Vet Inst Pulawy 2007, 51, 139-144.Search in Google Scholar

30. Nowak M., Madej. J.A., Dzięgiel P.: Correlation between the expression of HER2 receptor and Ki-67 antigen in mammary adenocarcinomas in bitches. Bull Vet Inst Pulawy 2005, 49, 337-342.Search in Google Scholar

31. Orlow I., Lacombe L., Hannon G.J., Serrano M., Pellicer I., Dalbagni G., Reuter V.E., Zhang Z.F., Beach D., Cordon-Cardo C.: Deletion of the p16 and p15 genes in human bladder tumors. J Natl Cancer Inst 1995, 87, 1524-1529.Search in Google Scholar

32. Pass D.A., Huxtable C.R., Cooper B.J., Watson A.D.J., Thompson R.: Canine laryngeal oncocytomas. Vet Pathol 1980, 17, 672-677.Search in Google Scholar

33. Rocco J.W., Sidransky D.: p16(MTS-1/CDKN2/INK4a) in cancer progression. Exp Cell Res 2001, 264, 42-55.Search in Google Scholar

34. Rocco A., Schandl L., Nardone G., Tulassay Z., Staibano S., Malfertheiner P., Ebert M.: Loss of expression of tumor suppressor p16(INK4) protein in human primary gastric cancer is related to the grade of differentiation. Dig Dis 2002, 20, 102-105.Search in Google Scholar

35. Rotstein D.S., Govett P., Wolfe B.Ł Laryngeal squamous cell carcinoma in a North American black bear (Ursus americanus). J Zoo Wildl Med 2005, 36, 543-545.Search in Google Scholar

36. Saik J.E., Toll S.L., Diters R.W., Goldschmidt M.H.: Canine and feline laryngeal neoplasia: a 10-year survey. J Am Anim Hosp Assoc 1986, 22, 359-365.Search in Google Scholar

37. Sapierzyński R.: Practical oncology in dogs and cats. Edited by Elsevier, Wroclaw, 2010, pp. 79-85.Search in Google Scholar

38. Schwarz P.D., Willer R.I.: Urinary bladder neoplasia in the dog and cat. Prob Vet Med 1989, 168, 425-427.Search in Google Scholar

39. Silva S.D., Nonogaki S., Soares F.A., Kowalski L.P.: p16 (INK4a) has clinicopathological and prognostic impact on oropharynx and larynx squamous cell carcinoma. Braz J Med Biol Res 2012, 45, 1327-1333.Search in Google Scholar

40. Smith E.M., Rubenstein L.M., Hoffman H., Haugen T.H., Turek L.P.: Human papillomavirus, p16 and p53 expression associated with survival of head and neck cancer. Infect Agent Cancer 2010, 5, 4-13.Search in Google Scholar

41. Stachura J., Domagała W.: Pathology - word about the disease. Vol II, PAU, Kraków, 2005, pp. 716-722.Search in Google Scholar

42. Strafuss A.C., Cook J.E., Smith J.E.: Squamous cell carcinoma in dogs. J Am Vet Med Assoc 1976, 168, 425-427.Search in Google Scholar

43. Walter J.H.: Cytokeratins in the canine epidermis. Vet Dermatol 2001, 12, 81-87.Search in Google Scholar

44. Weinberger P.M., Yu Z., Haffty B.G., Kowalski D., Harigopal M., Sasaki C., Rimm D.L., Psyrri A.: Prognostic significance of p16 protein levels in oropharyngeal squamous cell cancer. Clin Cancer Res 2004, 10, 5684-5691. Search in Google Scholar

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