1. bookVolume 11 (2018): Issue 4 (December 2018)
Journal Details
License
Format
Journal
eISSN
1337-9569
First Published
19 Jun 2009
Publication timeframe
4 times per year
Languages
English
access type Open Access

Alteration in MDA, GSH level and hematological changes due to thiamine deficiency in Mus musculus

Published Online: 18 Oct 2019
Volume & Issue: Volume 11 (2018) - Issue 4 (December 2018)
Page range: 321 - 325
Received: 16 Jun 2017
Accepted: 17 Mar 2018
Journal Details
License
Format
Journal
eISSN
1337-9569
First Published
19 Jun 2009
Publication timeframe
4 times per year
Languages
English
Abstract

It is known that thiamine deficiency may lead to Alzheimer’s diseases in humans. The present study has thus been conducted to understand the role of thiamine deficiency with respect to alteration in the peripheral blood of Swiss albino mice. For this purpose, adult Swiss albino mice (6–8 week old) were divided into three groups. The first group was control; the second (group II) and the third group (group III) were made thiamine deficient for 08 and 10 days respectively. Thiamine deficiency was induced in mice by injecting pyrithiamine (5 µg/10 g bwt) and feeding a thiamine deficient diet. The erythrocytes, leukocytes count, hemoglobin, hematocrit value, mass cell volume, mean corpuscular hemoglobin in blood of mice were determined by hematoanalyzer. Malondialdehyde (MDA) and reduced glutathione (GSH) level was also determined in serum of treated and non-treated groups. A significant reduction in leukocyte and erythrocyte count was observed in both the thiamine deficient groups as compared to control. Levels of hemoglobin and hematocrit value were also declined in the thiamine deficient groups. Enhancement in mass cell volume (MCV) level and decline in mean corpuscular hemoglobin (MCH) levels were observed in both thiamine deficient groups with respect to control. Inter-group comparison of all parameters also showed a significant value at p<0.01. In comparison with the control group, elevation in MDA and decline in GSH level was observed in both thiamine deficient groups which were statistically significant. These data indicate that thiamine deficiency leads to significant alterations in the hematological parameters as well as in MDA and GSH level.

Keywords

Agarwal S, Chaudhary B, Bist R. (2016). Bacoside A and bromelain relieve dichlorvos induced changes in oxidative responses in mice serum. Chemicobiological Interactions254: 173–178.Search in Google Scholar

Ashton N. (2013). Physiology of red and white blood cells. Anaesth Intensive Care14(6): 261–266.Search in Google Scholar

Badraoui R, Abdelmoula NB, Rebai T. (2011) Erythrocytes oxidative damage and hematological effects of 2, 4, 4 5-tetrachlorodiphenyl sulfone in rats. Experimental and Toxicologic Pathology63(5): 479–482.10.1016/j.etp.2010.03.00820382005Search in Google Scholar

Blokhina O, Virolainen E, Fagerstedt KV. (2003). Antioxidants, oxidative damage and oxygen deprivation stress: a review. Annals of Botany91(2): 179–194.Search in Google Scholar

Chineke CA, Ologun AG, Ikeobi CON. (2006). Haematological parameters in rabbit breeds and crosses in humid tropics. Pak J Biol Sci.9(11): 2102–2106.Search in Google Scholar

Ellman GL. (1959). Tissue sulfhydryl groups. Arch Biochem Biophys82: 70–77.Search in Google Scholar

Eze JI, Onunkwo JI, Shoyinka SVO, Chah FK, Ngene AA, Okolinta N, Onyenwe IW. (2015). Haematological profiles of pigs raised under intensive management system in South-Eastern Nigeria. Nigerian Veterinary Journal31(2): 115–123.Search in Google Scholar

Falahatkar B, Akhavan SR, Poursaeid S, Hasirbaf E. (2014). Use of sex steroid profiles and hematological indices to identify perinucleolus and migratory gonadal stages of captive Siberian sturgeon Acipenser baerii (Brandt, 1869) females. Journal of Applied Ichthyology30(6): 1578–1584.Search in Google Scholar

Ferreiro E, Baldeiras I, Ferreira IL, Costa RO, Rego AC, Pereira CF, Oliveira CR. (2012). Mitochondrial-and endoplasmic reticulum-associated oxidative stress in Alzheimer’s disease: from pathogenesis to biomarkers. International Journal of Cell Biology2012: 1–23.Search in Google Scholar

Fibach E, Rachmilewitz E. (2008). The role of oxidative stress in hemolytic anemia. Current Molecular Medicine8(7): 609–619.Search in Google Scholar

Fitzsimons JD, Williston B, Amcoff P, Balk L, Pecor C, Ketola HG, Honeyfield DC. (2005). The effect of thiamine injection on upstream migration, survival, and thiamine status of putative thiamine-deficient coho salmon. Journal of Aquatic Animal Health17(1): 48–58.Search in Google Scholar

Halliwell B, Gutteridge JMC. (1985). Oxygen radicals and the nervous system. Trends in Neurosciences8: 22–26.Search in Google Scholar

Hazell AS. (2009). Astrocytes are a major target in thiamine deficiency and Wernicke’s encephalopathy. Neurochemistry International55(1): 129–135.Search in Google Scholar

Hazell AS, Butterworth RF. (2009) Update of cell damage mechanisms in thia-mine deficiency: focus on oxidative stress, excitotoxicity and inflammation. Alcohol & Alcoholism44(2): 141–147.Search in Google Scholar

He Z, Sun Z, Liu S, Zhan, Q, Tan Z. (2009). Effects of early malnutrition on mental system, metabolic syndrome, immunity and the gastrointestinal tract. Journal of Veterinary Medical Science71(9): 1143–1150.Search in Google Scholar

Hirsch JA, Parrott J. (2012). New considerations on the neuromodulatory role of thiamine. Pharmacology.89(1–2): 111–116.Search in Google Scholar

Isaac LJ, Abah G, Akpan B, Ekaett, IU (2013) Haematological properties of different breeds and sexes of rabbits. In Proceedings of the 18th Annual Conference of Animal Science Association of Nigeria, 24–27.Search in Google Scholar

Jhala SS, Hazell AS. (2011). Modeling neurodegenerative disease pathophysiology in thiamine deficiency: consequences of impaired oxidative metabolism. Neurochemistry International58(3): 248–260.Search in Google Scholar

Johnston JK, Morris DD (1996) Alterations in blood proteins. In B. P. Smith (Ed.), International Animal Medicine (2nd ed.). USA: Mosby Publishers.Search in Google Scholar

Jozefczak M, Remans T, Vangronsveld J, Cuypers A. (2012). Glutathione is a key player in metal-induced oxidative stress defenses. International Journal of Molecular Sciences13(3): 3145–3175.Search in Google Scholar

Karuppagounder SS, Xu H, Shi Q, Chen LH, Pedrin, S, Pechman D, Gibson GE. (2009). Thiamine deficiency induces oxidative stress and exacerbates the plaque pathology in Alzheimer’s mouse model. Neurobiology of Aging30(10): 1587–1600.Search in Google Scholar

Ketola HG, Isaacs GR, Robins JS, Lloyd RC. (2008). Effectiveness and retention of thiamine and its analogs administered to steelhead and landlocked Atlantic salmon. Journal of Aquatic Animal Health20(1): 29–38.Search in Google Scholar

Kidd PM. (1997). Glutathione: systemic protectant against oxidative and free radical damage. Altern Med Rev2(3): 155–176.Search in Google Scholar

Koubkova M, Haertlova H, Knizkova I, Kunc P, Flusser J, Dolezal O. (2002). Influence of high environmental temperatures and evaporative cooling on some physiological, hematological and biochemical parameters in high-yielding dairy cows. Czech Journal of Animal Science-UZPI47(8): 309–318.Search in Google Scholar

Kronfeld DS, Medway W. (1969). In A textbook of veterinary clinical pathology. Edited by W. Medway, J. E. Prier, and J. S. Wilkinson. The Williams and Wilkins Co., Baltimore.Search in Google Scholar

Lakshmanan SA, Rajendran CS. (2013). Impact of Dichlorvos on tissue glycogen and protein content in freshwater fingerlings, Oreochromismossambicus (Peters). International Journal of Research in Environmental Science and Technology3(1): 19–25.Search in Google Scholar

Liu D, Ke Z, Luo J. (2017). Thiamine deficiency and neurodegeneration: the interplay among oxidative stress, endoplasmic reticulum stress, and autophagy. Molecular Neurobiology54(7): 5440–5448.Search in Google Scholar

Nasyrova DI, Sapronova AY, Nigmatullina RR, Ugrumov MV. (2006) Changes in blood plasma volume in rats during ontogenesis. Russian Journal of Developmental Biology37(5): 301–305.10.1134/S1062360406050031Search in Google Scholar

Ohkawa H, Ohshi N, Yagi K. (1979). Assay or lipid peroxides inanimal tissues by thiobarbituric acid reaction. Anal Biochem95: 351–58.Search in Google Scholar

Omiyale CA, Yisa AG, Ali-Dankrah LA. (2012). Haematological characteristics of Yankasa sheep fed Fonio (Digitariaiburua) straw based diets. In Proceeding of the 37th Annual Conference of the Nigerian Society for Animal Production37: 108–110.Search in Google Scholar

Reznick AZ, Packer L. (1993) Free radicals and antioxidants in muscular and neurological diseases and Disorders. In Free radicals: from basic science to Medicine 425–437.10.1007/978-3-0348-9116-5_37Search in Google Scholar

Sattar A, Mirza RH. (2009) Haematological parameters in exotic cows during gestation and lactation under subtropical conditions. Pakistan Veterinary Journal29(3): 129–132.Search in Google Scholar

Shangari N, Bruce WR, Poon R, O’brien, PJ. (2003). Toxicity of glyoxals–role of oxidative stress, metabolic detoxification and thiamine deficiency. Biochemical Society Transactions31(Pt 6): 1390–1393.Search in Google Scholar

Sharma A, Bis R. (2014). Thiamine deprivation disturbs cholinergic system and oxidative stress in liver of Mus musculus. Int J Pharmacol Pharm Sci6: 139–143.Search in Google Scholar

Sharma A, Bist R, Bubber P. (2013). Thiamine deficiency induces oxidative stress in brain mitochondria of Mus musculus. Journal of Physiology and Biochemistry69(3): 539–546.Search in Google Scholar

Sheu KF, Calingasan NY, Lindsay JG, Gibson GE. (1998). Immunochemical characterization of the deficiency of the alpha ketoglutarate dehydrogenase complex in thiamine-deficient rat brain. J Neurochem70(3): 1143–1150.Search in Google Scholar

Smithline HA, Donnino M, Greenblatt DJ. (2012). Pharmacokinetics of high-dose oral thiamine hydrochloride in healthy subjects. BMC Clinical Pharmacology12(1): 4.Search in Google Scholar

Soetan KO, Akinrinde AS, Ajibade TO.(2013). Preliminary studies on the haematological parameters of cockerels fed raw and processed guinea corn (Sorghum bicolor). In Proceedings of 38th Annual Conference of Nigerian Society for Animal Production pp. 49–52.Search in Google Scholar

Stohs SJ. (1990). Oxidative stress induced by 2, 3, 7, 8-tetrachlorodibenzo-pdioxin (TCDD). Free Radical Biology and Medicine9(1): 79–90.Search in Google Scholar

Tejashwini M, Padma MC. (2015). Counting of RBC’s and WBC’s Using Image Processing Technique. International. Journal. on Recent. and Innovation. Trends. in Computing. and Communication3(5): 2948–2953.Search in Google Scholar

Ugwuene MC. (2011). Effect of dietary palm kernel meal for maize on the haematological and serum chemistry of broiler turkey. Nigerian. Journal. of Animal. Science13: 93–103.Search in Google Scholar

Urso ML, Clarkson PM. (2003). Oxidative stress, exercise and antioxidant supplementation. Toxicology189(1): 41–54.Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo