1. bookVolume 45 (2022): Issue 1 (March 2022)
Journal Details
License
Format
Journal
eISSN
1857-7415
First Published
08 Sep 2014
Publication timeframe
2 times per year
Languages
English
access type Open Access

The Impact of Cattle Hide Cleanliness Scores on Microbial Contamination of Carcasses During Slaughtering

Published Online: 29 Mar 2022
Volume & Issue: Volume 45 (2022) - Issue 1 (March 2022)
Page range: 43 - 51
Received: 28 Jul 2021
Accepted: 20 Jan 2022
Journal Details
License
Format
Journal
eISSN
1857-7415
First Published
08 Sep 2014
Publication timeframe
2 times per year
Languages
English
Abstract

This study aimed to investigate the effects of cleanliness scoring on the microbiological load of hide and the final contamination of cattle carcasses. Fifty cattle were classified from 1 (clean and dry) to 5 (filthy and wet). Aerobic colony count (ACC) and counts of Enterobacteriaceae (EC) and E. coli (ECC) were determined on the brisket, abdominal midline, rump, groin sites of the hides, and brisket, flank, groin, and hock of the carcasses. On hides, ACC ranged from 3.15±0.13 log cfu/cm2 in category 1 to 8.14±0.21 log cfu/cm2 in category 5. EC and ECC were ranging between 1.13±0.07 and 2.80±0.09 log cfu/cm2, and 1.21±0.05 and 2.15±0.07 log cfu/cm2, respectively. While the mean ACC on the carcasses ranged between 2.18±0.07 and 2.63±0.05 log cfu/cm2 irrespective of the categories, Enterobacteriaceae and E. coli could not be counted due to the detection limits. It was concluded that although the level of bacterial load increased significantly (P<0.001) with the increasing cleanliness category on the hide of the animals, the reflection of this increasing trend on carcasses and different parts of the carcasses were inconsistent and the hygiene provided in the slaughterhouse and processing line was the main factor to reduce cross-contamination during processing.

Keywords

1. Antic, D., Blagojevic, B., Ducic, M., Mitrovic, R., Nastasijevic, I., Buncic, S. (2010). Treatment of cattle hides with Shellac-in-ethanol solution to reduce bacterial transferability - A preliminary study. Meat Sci. 85(1): 77-81. https://doi.org/10.1016/j.meatsci.2009.12.007 PMid:2037486810.1016/j.meatsci.2009.12.00720374868Search in Google Scholar

2. Obaidat, M.M. (2020). Prevalence and antimicrobial resistance of Listeria monocytogenes, Salmonella enterica and Escherichia coli O157:H7 in imported beef cattle in Jordan. Comp Immunol Microb Infect Dis. 70, 101447. https://doi.org/10.1016/j.cimid.2020.101447 PMid:3210583610.1016/j.cimid.2020.10144732105836Search in Google Scholar

3. Gill, C.O. (1998). Microbiological contamination of meat during slaughter and butchering of cattle, sheep and pigs. Microbiol Meat Poult. 118-157.Search in Google Scholar

4. Zweifel, C., Capek, M., Stephan, R. (2014). Microbiological contamination of cattle carcasses at different stages of slaughter in two abattoirs. Meat Sci. 98(2): 198-202. https://doi.org/10.1016/j.meatsci.2014.05.029 PMid:2496753910.1016/j.meatsci.2014.05.02924967539Search in Google Scholar

5. Cartier, P. (1994). Use of the ownership status of large cattle as an indicator of the microbial load of their leather. Meat and Meat Products 15(4): 119-122. [in French]Search in Google Scholar

6. Serraino, A., Bardasi, L., Riu, R., Pizzamiglio, V., Liuzzo, G., Galletti, G., Giacometti, F., Merialdi, G. (2012). Visual evaluation of cattle cleanliness and correlation to carcass microbial contamination during slaughtering. Meat Sci. 90(2): 502-506. https://doi.org/10.1016/j.meatsci.2011.08.001 PMid:2190688910.1016/j.meatsci.2011.08.00121906889Search in Google Scholar

7. Blagojevic, B., Antic, D., Ducic, M., Buncic, S. (2012). Visual cleanliness scores of cattle at slaughter and microbial loads on the hides and the carcases. Vet Rec. 170(22): 563. https://doi.org/10.1136/vr.100477 PMid:2250524410.1136/vr.10047722505244Search in Google Scholar

8. Van Donkersgoed, J., Jericho, K.W.F., Grogan, H., Thorlakson, B. (1997). Preslaughter hide status of cattle and the microbiology of carcasses. J Food Prot. 60(12): 1502-1508. https://doi.org/10.4315/0362-028X-60.12.1502 PMid:3120774610.4315/0362-028X-60.12.150231207746Search in Google Scholar

9. Antic, D., Blagojevic, B., Ducic, M., Nastasijevic, I., Mitrovic, R., Buncic, S. (2010). Distribution of microflora on cattle hides and its transmission to meat via direct contact. Food Control 21(7): 1025-1029. https://doi.org/10.1016/j.foodcont.2009.12.02210.1016/j.foodcont.2009.12.022Search in Google Scholar

10. Buncic, S., Nychas, G.J., Lee, M.R.F., Koutsoumanis, K., Hébraud, M., Desvaux, M., Chorianopoulos, N., et al. (2014). Microbial pathogen control in the beef chain: Recent research advances. Meat Sci. 97(3): 288-297. https://doi.org/10.1016/j.meatsci.2013.04.040 PMid:2368879710.1016/j.meatsci.2013.04.04023688797Search in Google Scholar

11. Food Standards Agency [Internet]. Red meat safety & clean livestock. Published by the Food Standards Agency June 2002 [Reprinted March 2004]. https://www.foodstandards.gov.scot/downloads/Red_meat_safety_and_clean_livestock.pdfSearch in Google Scholar

12. European Commission (2004) [Internet]. Regulation (EC) N° 852/2004 of the European Parliament and of the Council of 29 April 2004 on the hygiene of foodstuffs. Official Journal of the European Union, L 139(1), 54. https://eur-lex.europa.eu/eli/reg/2004/852/ojSearch in Google Scholar

13. European Commission (2004) [Internet]. Regulation (EC) N° 853/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules for on the hygiene of foodstuffs. Official Journal of the European Union, L 139(853), 55. https://eur-lex.europa.eu/legal-content/en/ALL/?uri=CELEX%3A32004R0853Search in Google Scholar

14. Food Standards Agency [Internet]. Clean beef cattle for slaughter. A guide for producers. Published by the Food Standard Agency October 2007. https://www.foodstandards.gov.scot/downloads/cleanbeefsaf1007.pdfSearch in Google Scholar

15. AFSCA (2006) [Internet]. Scientific committee of the federal agency for the safety of the food chain. 2006. D/2006/10413/2 - Good condition of the fleece for sour meat. Bruxelles: Gil Houins, 2006b. [in French] https://www.favv-afsca.be/publicationsthematiques/_documents/Folder_VC_FR_26-01-2018_web.pdfSearch in Google Scholar

16. Bastien, D., Lucbert, J., Cartier, P. (2006). Cattle cleanliness at slaughter: observations report, factors involved and a method to grade. Renc Rech Ruminants. 13, 317-320. [in French]Search in Google Scholar

17. Regulation of special hygiene rules for animal food. Law of authorization: 5996. Official gazette of publication: 27.12.2011. Nr. 28155.Search in Google Scholar

18. Cenci-Goga, B.T., Miraglia, D., Ranucci, D., Branciari, R., Budelli, L., McCrindle, C.M., Cioffi, A., Mammoli, R. (2007). An in vitro system for the comparison of excision and wet-dry swabbing for microbiological sampling of beef carcasses. J Food Prot. 70(4): 930-936. https://doi.org/10.4315/0362-028X-70.4.930 PMid:1747726310.4315/0362-028X-70.4.93017477263Search in Google Scholar

19. ISO-ISO 4833-2:2013 [Internet]. Microbiology of the food chain - Horizontal method for the enumeration of microorganisms - Part 2: Colony count at 30 degrees C by the surface plating tech-nique. International Standard 2013, Geneva, Switzerland. [Retrieved February 19, 2021]. https://www.iso.org/standard/29824.htmlSearch in Google Scholar

20. ISO - ISO 21528-2:2004 [Internet]. Microbiology of food and animal feeding stuffs -Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 2: Colony-count method. [Retrieved February 19, 2021]. https://www.iso.org/standard/34566.htmlSearch in Google Scholar

21. ISO - ISO 16649-2:2001 [Internet]. Microbiology of food and animal feeding stuffs -horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide. [Retrieved February 19, 2021]. https://www.iso.org/standard/29824.htmlSearch in Google Scholar

22. Barco, L., Belluco, S., Roccato, A., Ricci, A. (2015). A systematic review of studies on Escherichia coli and Enterobacteriaceae on beef carcasses at the slaughterhouse. Int J Food Microbiol. 207, 30-39. https://doi.org/10.1016/j.ijfoodmicro.2015.04.027 PMid:2597880310.1016/j.ijfoodmicro.2015.04.02725978803Search in Google Scholar

23. ICMSF (International Commission on Microbiological Specifications for Foods) (2000). Microorganisms in foods 6: Microbial ecology of food commodities. Maryland: Aspen Publishers Inc.Search in Google Scholar

24. Nastasijevic, I., Tomasevic, I., Smigic, N., Milicevic, D., Petrovic, Z., Djekic, I. (2016). Hygiene assessment of Serbian meat establishments using different scoring systems. Food Control 62, 193-200. https://doi.org/10.1016/j.foodcont.2015.10.03410.1016/j.foodcont.2015.10.034Search in Google Scholar

25. Hauge, S., Nafstad, O., Røtterud, O.J., Nesbakken, T. (2012). The hygienic impact of categorisation of cattle by hide cleanliness in the abattoir. Food Control 27(1): 100-107. https://doi.org/10.1016/j.foodcont.2012.03.00410.1016/j.foodcont.2012.03.004Search in Google Scholar

26. Johanson, L., Underdal, B., Grøsland, K., Whelehan, O.P., Roberts, T.A. (1983). A survey of the hygienic quality of beef and pork carcasses in Norway. Acta Vet Scand. 24(1): 1-13. https://doi.org/10.1186/BF03546753 PMid:6869140 PMCid:PMC829125510.1186/BF03546753Search in Google Scholar

27. Arthur, T.M., Bosilevac, J.M., Nou, X., Shackelford, S.D., Wheeler, T.L., Kent, M.P., Jaroni, D., et al. (2004). Escherichia coli O157 prevalence and enumeration of aerobic bacteria, Enterobacteriaceae, and Escherichia coli O157 at various steps in commercial beef processing plants. J Food Prot. 67(4): 658-665. https://doi.org/10.4315/0362-028X-67.4.658 PMid:1508371510.4315/0362-028X-67.4.658Search in Google Scholar

28. Reid, C.A., Small, A., Avery, S.M., Buncic, S. (2002). Presence of food-borne pathogens on cattle hides. Food Control 13(6-7): 411-415. https://doi.org/10.1016/S0956-7135(01)00050-010.1016/S0956-7135(01)00050-0Search in Google Scholar

29. Garcia, A.B., Steele, W.B., Reid, S.W.J., Taylor, D.J. (2010). Risk of carcass contamination with Campylobacter in sheep sent for slaughter into an abattoir in Scotland. Prev Vet Med. 95(1-2): 99-107. https://doi.org/10.1016/j.prevetmed.2010.03.008 PMid:2038118110.1016/j.prevetmed.2010.03.008Search in Google Scholar

30. Jericho, K.W.F., Bradley, J.A., Gannon, V.P.J., Kozub, G.C. (1993). Visual demerit and microbiological evaluation of beef carcasses: Methodology. J Food Prot. 56(2): 114-119. https://doi.org/10.4315/0362-028X-56.2.114 PMid:3108411710.4315/0362-028X-56.2.114Search in Google Scholar

31. Mackey, B.M., Roberts, T.A. (1993). Improving slaughter hygiene using HACCP and monitoring. Fleischwirtschaft Int. 2, 40-45.Search in Google Scholar

32. Reneau, J.K., Seykora, A.J., Heins, B.J. (2003). Relationship of cow hygiene scores and SCC. Proc Natl Mast Coun. Madison, WI. 362-363.Search in Google Scholar

33. Ruud, L.E., Bøe, K.E., Østerås, O. (2010). Risk factors for dirty dairy cows in Norwegian freestall systems. J Dairy Sci. 93(11): 5216-5224. https://doi.org/10.3168/jds.2010-3321 PMid:2096533610.3168/jds.2010-3321Search in Google Scholar

34. Ridell, J., Korkeala, H. (1993). Special treatment during slaughtering in Finland of cattle carrying an excessive load of dung; Meat hygienic aspects. Meat Sci. 35(2): 223-228. https://doi.org/10.1016/0309-1740(93)90052-J10.1016/0309-1740(93)90052-JSearch in Google Scholar

35. Alvseike, O., Røssvoll, E., Røtterud, O.J., Nesbakken, T., Skjerve, E., Prieto, M., Sandberg, M., et al. (2019). Slaughter hygiene in European cattle and sheep abattoirs assessed by microbiological testing and hygiene performance rating. Food Control 101, 233-240. https://doi.org/10.1016/j.foodcont.2019.01.03310.1016/j.foodcont.2019.01.033Search in Google Scholar

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