1. bookVolume 59 (2014): Issue 3 (August 2014)
Journal Details
License
Format
Journal
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English
access type Open Access

The relationship between the alkaline phosphatase network and the haematopoiesis in mice subjected to whole-body irradiation

Published Online: 12 Sep 2014
Page range: 105 - 110
Received: 20 Feb 2014
Accepted: 11 Jun 2014
Journal Details
License
Format
Journal
First Published
25 Mar 2014
Publication timeframe
4 times per year
Languages
English

Purpose: To investigate the relationship between the alkaline phosphatase (ALP) network of the marrow stroma and the haematopoietic regeneration after mice whole-body irradiation. Materials and methods: Three groups of mice were irradiated with a non-lethal ionising radiation dose: the fi rst one received an intraperitoneal injection of Levamisole, ALP inhibitor, 24 h before irradiation; the second one received an intraperitoneal injection of Lisinopril, haematopoiesis inhibitor, 24 h before irradiation; the third was left untreated, but irradiated. The fourth group, untreated and not irradiated, was the control. The total surface occupied by ALP positive processes, revealed by means of ALP cytochemistry in the marrow area, was evaluated semi-quantitively. Nucleated bone marrow cells were also counted. Results: ALP network began to increase 24 h after irradiation to reach a maximum after 72 h, when the bone marrow was almost become completely empty of the haematopoietic cells. This increase advances the haematopoietic recovery. This process was substantially delayed when the mice were injected with Levamisole 24 h before irradiation. On the contrary, ALP network increased strongly since the fi rst day after irradiation when the mice were injected with Lisinopril 24 h before irradiation. Conclusions: These data have indicated that the haematopoietic recovery and repopulation of the bone marrow were advanced by the ALP network recovery.

Keywords

1. Mayani, H., Guilbert, L., & Janowska-Wieczorek, A. (1992). Biology of the hemopoietic microenvironment. Eur. J. Haematol., 49, 225-233.Search in Google Scholar

2. Bianco, P., & Boyde, A. (1993). Confocal images of marrow stromal (Westen-Bainton) cells. Histochemistry, 100, 93-99.Search in Google Scholar

3. Kittler, E. L., McGrath, H., Temeles, D., Crittenden, R. B., Kister, V. K., & Quesenberry, P. J. (1992). Biologic signifi cance of constitutive and subliminal growth factor production by bone marrow stroma. Blood, 79, 3168-3178.Search in Google Scholar

4. Long, H. W. (1992). Blood cell cytoadhesion molecules. Exp. Hematol., 20, 288-301.Search in Google Scholar

5. Krenacs, T., & Rosendaal, M. (1998). Connexin 43 gap junctions in normal, regenerating and cultured mouse bone marrow and in human leukemia: their possible involvement in blood formation. Am. J. Pathol., 152, 993-1004.Search in Google Scholar

6. Westen, H., & Baiton, D. F. (1979). Association of alkaline-phosphatase-reticulum cells in bone marrow with granulocytic precursors. J. Exp. Med., 150, 919-937.Search in Google Scholar

7. McKenna, R. W., & Allison, P. (1990). Diagnosis, classifi cation, and course of myelodysplasic syndromes. Clin. Lab. Med., 10, 683-706.Search in Google Scholar

8. Bianco, P., Bradbeer, J. N., Riminucci, M., & Boyde, A. (1993). Marrow stromal (Western-Bainton) cells: identifi cation, morphometry, confocal imaging and changes in disease. Bone, 14, 315-320.Search in Google Scholar

9. Dilly, S. A., & Jagger, C. J. (1990). Bone marrow stromal cell changes in haematological malignancies. J. Clin. Pathol., 43, 942-947.Search in Google Scholar

10. Almohamad, K., Thiry, A., Hubin, F., Belaid, Z., Humblet, C., Boniver, J., & Defresne, M. -P. (2003). Marrow stromal cell recovery after radiation-induced aplasia in mice. Int. J. Radiat. Biol., 79(4), 259-267.Search in Google Scholar

11. Rousseau-Plasse, A., Wdzieczak-Bakala, J., Lenfant, M., Ezan, E., Robinson, S., Briscoe, C. V., Melville, J., & Riches, A. (1998). Lisinopril, an angiotensin I-converting enzyme inhibitor, prevents entry of murine hematopoietic stem cells into the cell cycle after irradiation in vivo. Exp. Hematol., 26, 1074-1079.Search in Google Scholar

12. Fallon, M. D., Whyte, M. P., & Teitelbaum, S. L. (1980). Stereospecifi c inhibition of alkaline phosphatase by L-tetramisole prevents in vitro cartilage calcifi cation. Lab. Invest., 43, 489-494.Search in Google Scholar

13. Scher, B. M., Fuksina, I., Hellinger, N., Waxman, S., & Scher, W. (1998). The phosphatase inhibitors, orthovanadate and levamisole, inhibit induction of erythroid differentiation and abrogate the associated inhibition of glycolysis. Int. J. Oncol., 12, 987-996.Search in Google Scholar

14. Bianco, P., Costantini, M., Dearden, L. C., & Bonucci, E. (1988). Alkaline phosphatase positive precursors of adipocytes in the human bone marrow. Br. J. Haematol., 68, 401-403.Search in Google Scholar

15. Prince, M. H., Simmons, P. J., Whitty, G., Wall, D. P., Barber, L., Toner, G. C., Seymoour, R. F., Richardson, G., Mrongovius, R., & Haylock, D. N. (2004). Improved hematopoietic recovery following transplantation with ex vivo-expanded mobilized blood cells. Br. J. Haematol., 126(4), 536-545.Search in Google Scholar

16. Haot, J., & Barakina, N. F. (1969). Quantitative study of the hemopoietic recovery after a sublethal X-irradiation in the mouse. Acta Haematol., 42, 347-356.Search in Google Scholar

17. Imai, Y., & Nakao, I. (1987). In vivo radiosensitivity and recovery pattern of the hematopoietic precursor cells and stem cells in mouse bone marrow. Exp. Hematol., 15, 890-895.Search in Google Scholar

18. Brookoff, D., & Weiss, L. (1982). Adipocyte development and the loss of erythropoietic capacity in the bone marrow of mice after sustained hypertransfusion. Blood, 60, 1337-1344. Search in Google Scholar

19. Dilly, S. A., Jagger, C. J., & Sloane, J. P. (1993). Stromal cell populations in necropsy bone marrow sections from allogeneic marrow recipients and non- -transplant patients. J. Clin. Pathol., 46, 611-616.Search in Google Scholar

20. Poncin, G., Beaulieu, A., Humblet, C., Thiry, A., Oda, K., Boniver, J., & Defresne, M. -P. (2012). Characterization of spontaneous bone marrow recovery after sublethal total body irradiation: importance of the osteoblastic/adipocytic balance. PLoS One 7:e30818. Search in Google Scholar

Recommended articles from Trend MD

Plan your remote conference with Sciendo