1. bookVolume 26 (2018): Issue 4 (October 2018)
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08 Aug 2013
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access type Open Access

Evaluation of antioxidant, antimicrobial and cytotoxic potential in Artemisia vulgaris L.

Published Online: 29 Oct 2018
Page range: 431 - 441
Received: 04 Apr 2018
Accepted: 04 May 2018
Journal Details
License
Format
Journal
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English

Artemisia vulgaris L. (Mugwort or Afsantin) has been used to treat various diseases since ancient times by the inhabitants of Himalayan region-Pakistan. Methanolic fractions (HA1-HA9) obtained from the aerial parts of A. vulgaris were evaluated for their antioxidant, antimicrobial and brine shrimp cytotoxic activities. Fraction HA8 showed substantial phenolics content with value of 26.29±1.4μgEQ/mg and DPPH scavenging (82.84±3.01%). Conversely, total flavonoids content of 7.32±0.07μgEQ/mg was determined in HA1 fraction. Fraction HA1 also showed significant cytotoxic effect with the value LD50 of 144.94μg/mL. Fractions HA7 and HA9 depicted maximum total antioxidant activity and ferric ion reduction (96.25±3.29 and AAE/mg and 176.91±8, respectively). All fractions showed encouraging results against bacterial strains Bordetella bronchiseptica and Micrococcus luteus, while HA2 fraction showed the highest percentage inhibition Mucor species with zone of inhibition of 13.25±0.35mm. A total of 7 fractions showed significant antileishmanial activity with survival percentage ranging 0.00 to 19. To sum up, results of the current study indicated that the plant can be further explored for isolation of antileishmanial and antimicrobial compounds, which could be used for drug development.

Keywords

1. WHO (World Health Organization), Traditional and Alternative Medicine, Fact Sheet No 271. World Health Organization, Geneva 2002.Search in Google Scholar

2. Donehower RC, Rowinsky EK. An overview of experience with taxol (paclitaxel) in the USA. Canc Treatm Rev 1993; 19:63-8. DOI: 10.1016/0305-7372(93)90049-WSearch in Google Scholar

3. Huie CW. A review of modern sample-preparation techniques for the extraction and analysis of medicinal plants. Analyt Bioanalyt Chem 2002; 373(1-2): 23-30. DOI: 10.1007/s00216-002-1265-310.1007/s00216-002-1265-3Open DOISearch in Google Scholar

4. Nitta T, Arai T, Takamatsu H, Inatomi Y, Murata H, Iinuma M, et al. Antibacterial Activity of Extracts Prepared from Tropical and Subtropical Plants on Methicillin-Resistant Staphylococcus aureus. J Health Sci 2002; 48(3):273-6. DOI: 10.1248/jhs.48.27310.1248/jhs.48.273Open DOISearch in Google Scholar

5. Ivanova D, Gerova D, Chervenkov T, Yankova T. Polyphenols and antioxidant capacity of Bulgarian medicinal plants. J Ethnopharmacol 2005; 96(1):145-50. DOI: 10.1016/j.jep.2004.08.03310.1016/j.jep.2004.08.033Open DOISearch in Google Scholar

6. Costa ES, Hiruma LCA, Lima EO, Sucupira GC, Bertolin AO, Lolis SF, et al. Antimicrobial activity of some medicinal plants of the Cerrado, Brazil. Phytotherap Res 2008; 22(5):705-07. DOI: 10.1002/ptr.239710.1002/ptr.2397Open DOISearch in Google Scholar

7. Nair R, Chanda S. Activity of some medicinal plants against certain pathogenic bacterial strains. Ind J Pharmacol 2006; 38(2):142-4. DOI: 10.4103/0253-7613.2462510.4103/0253-7613.24625Open DOISearch in Google Scholar

8. Cowan MM. Plant products as antimicrobial agents. Clin Micro Rev 1999; 12(4);564-82.Search in Google Scholar

9. Ahmad I, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol 1998; 62(2):183-93. DOI: 10.1016/S0378-8741(98)00055-510.1016/S0378-8741(98)00055-5Open DOISearch in Google Scholar

10. Wright GD. Something old, something new: revisiting natural products in antibiotic drug discovery 1. Canad J Micro 2014;6 0(3):147-4Search in Google Scholar

11. Emori TG, Gaynes RP. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin Micro Rev 1993; 6(4):428-42. DOI: 10.1128/CMR.6.4.42810.1128/CMR.6.4.428Open DOISearch in Google Scholar

12. Pannuti CS, Grinbaum RS. An overview of nosocomial infection control in Brazil. Infec Control Hosp Epidem 1995; 16:170-4. DOI: 10.2307/3014096710.2307/30140967Open DOISearch in Google Scholar

13. Bartosz G. Reactive oxygen species: destroyers or messengers? Biochem Pharmacol 2009; 77(8):1303-15. DOI: 10.1016/j.bcp.2008.11.009Search in Google Scholar

14. Lenaz G. Mitochondria and reactive oxygen species. Which role in physiology and pathology? In: Scatena R, Bottoni P, Giardina B editors. Advances in mitochondrial medicine vol 942. Advances in Experimental Medicine and Biology, Springer Dordrecht, 2012; 93-136. DOI: 10.1007/978-94-007-2869-1_510.1007/978-94-007-2869-1_5Open DOISearch in Google Scholar

15. Becker LB. New concepts in reactive oxygen species and cardiovascular reperfusion physiology. Cardiovascul Res 2004;61(3):461-70. DOI: 10.1016/j.cardiores.2003.10.02510.1016/j.cardiores.2003.10.025Open DOISearch in Google Scholar

16. Halliwell B. Oxidative stress and cancer: have we moved forward? Biochem J 2007; 401(1):1-11. DOI: 10.1042/BJ2006113110.1042/BJ20061131Open DOISearch in Google Scholar

17. Harman D. The free radical theory of aging. Antioxidants and Redox Signaling 2003; 5(5):557-61. DOI: 10.1089/15230860377031020210.1089/152308603770310202Open DOISearch in Google Scholar

18. Jaeschke H. Reactive oxygen and mechanisms of inflammatory liver injury: present concepts. J Gastroenterol Hepatol 2011; 26(1):173-79. DOI: 10.1111/j.1440-1746.2010.06592.x10.1111/j.1440-1746.2010.06592.xOpen DOISearch in Google Scholar

19. Reed TT. Lipid peroxidation and neurodegenerative disease. Free Rad Biol Med 2011;51(7):1302-19. DOI: 10.1016/j.freeradbiomed.2011.06.02710.1016/j.freeradbiomed.2011.06.027Open DOISearch in Google Scholar

20. Venardos KM, Kaye DM. Myocardial ischemia-reperfusion injury, antioxidant enzyme systems, and selenium: a review. Curr Med Chem 2007; 14(14):1539-49. DOI: 10.2174/09298670778083107810.2174/092986707780831078Open DOISearch in Google Scholar

21. Bahorun T, Ramma AL, Amitabye, Alan C, Aruoma OI. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. J Sci Food Agricul 2004; 84(12):1553-61. DOI: 10.1002/jsfa.182010.1002/jsfa.1820Open DOISearch in Google Scholar

22. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958; 181:1199-00. DOI: 10.1038/1811199a0Search in Google Scholar

23. Halliwell B. Reactive oxygen species and the central nervous system Free Rad Brain 1992; 21-40.Search in Google Scholar

24. Rao, YK, Geethangili M, Fang SH, Tzeng YM. Antioxidant and cytotoxic activities of naturally occurring phenolic and related compounds: a comparative study. Food Chem Toxicol 2007; 45(9):1770-6. DOI: 10.1016/j.fct.2007.03.01210.1016/j.fct.2007.03.012Open DOISearch in Google Scholar

25. Krings U, Berger RG. Antioxidant activity of some roasted foods. Food Chem 2001; 72(2):223-9. DOI: 10.1016/S0308-8146(00)00226-010.1016/S0308-8146(00)00226-0Open DOISearch in Google Scholar

26. Sasidharan S, Chen Y, Saravanan D, Sundram KM, Latha LY. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med 2011; 8(1): 1-10Search in Google Scholar

27. Osawa, T. Novel natural antioxidants for utilization in food and biological systems. In I. Uritani, V. V. Garcia, & E. M. Mendoza Editors, Postharvest biochemistry of plant food-materials in the tropics Tokyo, Japan: Japan Scientific Societies Press pp. 241-51, 1994Search in Google Scholar

28. Tabti L, Mohammed ElAD, Gaouar N, Samira B, Tabti, B. Antioxidant and Antifungal Activity of Extracts of the Aerial Parts of Thymus capitatus (L.) Hoffmanns Against Four Phytopathogenic Fungi of Citrus sinensis. Jundishapur J Nat Pharm Prod 2014; 9(1):49-4. DOI: 10.17795/jjnpp-13972Search in Google Scholar

29. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analyt Biochem 1999; 269(2):337-41. DOI: 10.1006/abio.1999.401910.1006/abio.1999.4019Open DOISearch in Google Scholar

30. Braga FG, Bouzada ML, Fabri RL, DeOMatos M, Moreira FO, Scio E, et al. Antileishmanial and antifungal activity of plants used in traditional medicine in Brazil. J Ethnopharmacol 2007; 111(2):396-02. DOI: 10.1016/j.jep.2006.12.006Search in Google Scholar

31. Lu XL, Najafzadeh MJ, Dolatabadi S, Ran YP, Gerritisvanden AHG, Shen YN et al. Taxonomy and epidemiology of Mucor irregularis, agent of chronic cutaneous mucormycosis. Persoonia: Mol Phylog Evol Fung 2013; 30:48-56. DOI: 10.3767/003158513X66553910.3767/003158513X665539Open DOISearch in Google Scholar

32. Schuster E, Dunn CN, Frisvad J, Van DP. On the safety of Aspergillus niger - a review. Appl Micro Biotechnol 2002; 59(4-5):426-35. DOI: 10.1007/s00253-002-1032-610.1007/s00253-002-1032-6Open DOISearch in Google Scholar

33. Woolfrey BERTF, Moody JULIAA. Human infections associated with Bordetella bronchiseptica. Clin Micro Rev 1991; 4(3):243-55. DOI: 10.1128/CMR.4.3.24310.1128/CMR.4.3.243Open DOISearch in Google Scholar

34. Grimaldi G, Tesh RB. Leishmaniases of the New World: current concepts and implications for future research. Clin Micro Rev 1993; 6(3):230-50. DOI: 10.1128/CMR.6.3.23010.1128/CMR.6.3.230Open DOISearch in Google Scholar

35. Singh S, Sivakumar R. Challenges and new discoveries in the treatment of leishmaniasis. J Infec Chemothr 2004; 10(6):307-15. DOI: 10.1007/s10156-004-0348-910.1007/s10156-004-0348-9Open DOISearch in Google Scholar

36. Sundar S, Rai M. Laboratory diagnosis of visceral leishmaniasis. Clin Diagn Lab Immunol 2002; 9(5):951-8. DOI: 10.1128/CDLI.9.5.951-958.2002Search in Google Scholar

37. Beukelman CJ, Vanden WE, Henriette C, Van UQ, Kroes BH, Vanden BJ. Discovery of new anti-inflammatory drugs from plant origin. Annals Gastroenterol 2002; 15(4):320-3.Search in Google Scholar

38. Chohan ZH, Shaikh AU, Rauf A, Supuran CT. Antibacterial, antifungal and cytotoxic properties of novel N-substituted sulfonamides from 4-hydroxycoumarin. J Enz Inhibit Med Chem 2006; 21(6):741-8. DOI: 10.1080/14756360600810340Search in Google Scholar

39. Khan A, Gilani AH. Antispasmodic and bronchodilator activities of Artemisia vulgaris are mediated through dual blockade of muscarinic receptors and calcium influx. J Ethnopharmacol 2009; 126(3):480-6. DOI: 10.1016/j.jep.2009.09.01010.1016/j.jep.2009.09.010Open DOISearch in Google Scholar

40. Pires JM, Mendes FR, Negri G, Almeida DJM, Carlini EA. Antinociceptive peripheral effect of Achillea millefolium L. and Artemisia vulgaris L.: both plants known popularly by brand names of analgesic drugs. Phytother Res 2009; 23(2): 212-9. DOI: 10.1002/ptr.258910.1002/ptr.2589Open DOISearch in Google Scholar

41. Gilani AH, Yaeesh S, Jamal Q, Ghayur MN. Hepatoprotective activity of aqueous–methanol extract of Artemisia vulgaris. Phytother Res 2005; 19(2):170-2. DOI: 10.1002/ptr.163210.1002/ptr.1632Open DOISearch in Google Scholar

42. Clarke G, Ting KN, Wiart C, Fry J. High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the Malaysian rainforest. Antioxidants 2013;2(1):1-10. DOI: 10.3390/antiox201000110.3390/antiox2010001Open DOISearch in Google Scholar

43. Haq I, Ullah N, Bibi G, Kanwal S, Ahmad MS, Mirza B. Antioxidant and cytotoxic activities and phytochemical analysis of Euphorbia wallichii root extract and its fractions. Iran J Pharm Res 2012;11(1):241-9.Search in Google Scholar

44. Lee SE, Hwang HJ, Ha JS, Jeong HS, Kim JH. Screening of medicinal plant extracts for antioxidant activity. Life Sci 2003; 73(2):167-9. DOI: 10.1016/S0024-3205(03)00259-510.1016/S0024-3205(03)00259-5Open DOISearch in Google Scholar

45. Ullah N, Haq I, Safdar N, Mirza B. Physiological and biochemical mechanisms of allelopathy mediated by the allelochemical extracts of Phytolacca latbenia (Moq.) H. Walter. Toxicol Ind Health 2013; 31(10): 931-7. DOI: 10.1177/0748233713483205Search in Google Scholar

46. Zaidi MA, Crow SA. Biologically active traditional medicinal herbs from Balochistan, Pakistan. J Ethnopharmacol 2005; 96(1):331-4. DOI: 10.1016/j.jep.2004.07.02310.1016/j.jep.2004.07.023Open DOISearch in Google Scholar

47. Manavathu EK, Ramesh MS, Baskaran I, Ganesan LT, Chandrasekar PH. A comparative study of the post-antifungal effect (PAFE) of amphotericin B, triazoles and echinocandins on Aspergillus fumigatus and Candida albicans. J Antimicrob Chemother 2004; 53(2):386-89. DOI: 10.1093/jac/dkh06610.1093/jac/dkh066Open DOISearch in Google Scholar

48. Ma G, Khan SI, Jacob MR, Tekwani BL, Li Z, Pasco DS, et al. Antimicrobial and antileishmanial activities of hypocrellins A and B. Antimicrob Agents Chemother 2004; 48(11):4450-52. DOI: 10.1128/AAC.48.11.4450-4452.200410.1128/AAC.48.11.4450-4452.2004Open DOISearch in Google Scholar

49. Ali N, Shah I, Shah SWA, Ahmed G, Shoaib M, Junaid M, et al. Antioxidant and relaxant activity of fractions of crude methanol extract and essential oil of Artemisiamacrocephala jacquem. BMC Complement Alternat Med 2013; 13(1):96. DOI: 10.1186/1472-6882-13-9610.1186/1472-6882-13-96Open DOISearch in Google Scholar

50. Medini F, Fellah H, Ksouri R, Abdelly C. Total phenolic, flavonoid and tannin contents and antioxidant and antimicrobial activities of organic extracts of shoots of the plant Limonium delicatulum. J Taib Univ Sci 2014; 8(3):216-24. DOI: 10.1016/j.jtusci.2014.01.00310.1016/j.jtusci.2014.01.003Open DOISearch in Google Scholar

51. Ahameethunisa AR, Hopper W. Antibacterial activity of Artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria. BMC complement Alternat Med 2010; 10(1):6. DOI: 10.1186/1472-6882-10-610.1186/1472-6882-10-6Open DOISearch in Google Scholar

52. Mighri H, Ahmed HH, Najjaa AK, Neffati M. Antimicrobial and antioxidant activities of Artemisia herba-alba essential oil cultivated in Tunisian arid zone. Comptes Rendus Chimie 2010; 13(3): 380-6 DOI: 10.1016/j.crci.2009.09.00810.1016/j.crci.2009.09.008Open DOISearch in Google Scholar

53. Youssef MM, Mahmoud AA, Al-Faiyz YSS. Antimicrobial and Antioxidant Activities of Artemisia abyssinica Extracts and DNA Degradation Effects. Asian J. Biochem 2015;10(1):31-41. DOI: 10.3923/ajb.2015.31.4110.3923/ajb.2015.31.41Open DOISearch in Google Scholar

54. Matic NA, Bunce PE. Isolation of Bordetella bronchiseptica from blood and a pancreatic abscess. J. Clin. Microbiol 2015; 53:1778–80. DOI: 10.1128/JCM.00175-1510.1128/JCM.00175-15Open DOISearch in Google Scholar

55. Uso J, Gill M, Gomila B, Tirado MD. Endocarditis due to Micrococcus luteus. Microbiol Clin 2003; 21:116-7.Search in Google Scholar

56. Al Bayati FA. Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts. J Ethnopharmacol 2008;116(3):403-6. DOI: 10.1016/j.jep.2007.12.00310.1016/j.jep.2007.12.003Open DOISearch in Google Scholar

57. Mannan A, Inayatullah S, Akhtar MZ, Qayyum M, Mirza B. Biological evaluation of wild thyme (Thymus serpyllum). Pharm Biol 2009;47(7):628-33. DOI: 10.1080/1388020090291562210.1080/13880200902915622Open DOISearch in Google Scholar

58. Rocha LG, Almeida JRGS, Macedo RO, Barbosa FJM. A review of natural products with antileishmanial activity. Phytomed 2005;12(6):514-5. DOI: 10.1016/j.phymed.2003.10.00610.1016/j.phymed.2003.10.006Open DOISearch in Google Scholar

59. Addo-Mensah A, Garcia G, Maldonado IA, Anaya E, Cadena, G Lee LG. Evaluation of Antibacterial Activity of Artemisia vulgaris Extracts. Research J. Medic Plants 2015;9:234-40. DOI: 10.3923/rjmp.2015.234.24010.3923/rjmp.2015.234.240Open DOISearch in Google Scholar

60. Ihsan-ul-Haq A, Ahmed I, Hussain I, Jamil M, Mirza B. Antibacterial activity and brine shrimp toxicity of Artemisia dubia extract. Pakistan J. Bot 2012;44(4):1487-90.Search in Google Scholar

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