1. bookVolume 27 (2019): Issue 3 (July 2019)
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08 Aug 2013
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4 times per year
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access type Open Access

Magnesium isoglycyrrhizinate protects against concanavalin A-induced immunological liver injury in a mouse model

Published Online: 30 Jul 2019
Page range: 281 - 290
Received: 15 Oct 2018
Accepted: 11 Apr 2019
Journal Details
License
Format
Journal
First Published
08 Aug 2013
Publication timeframe
4 times per year
Languages
English

Background: To evaluate the protective effects of magnesium isoglycyrrhizinate on a mouse model of concanavalin A (ConA)-induced immunological liver injury. Materials and Methods: Forty-eight mice were randomly divided into a normal control group, a model group, three dose groups of magnesium isoglycyrrhizinate (12.5, 25, 50 mg/kg) and a dexamethasone group (2.5 mg/kg). Magnesium isoglycyrrhizinate was intraperitoneally injected for 5 consecutive days, and the model of immunological liver injury was established on the fifth day after caudal vein injection of ConA (20 mg/kg). Blood was collected to detect the activities of alanine transaminase (ALT) and aspartate transaminase (AST) as well as the levels of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ). The levels of neopterin (NP) and malondialdehyde (MDA) and the activities of myeloperoxidase (MPO) and superoxide dismutase (SOD) in liver tissues were measured, and histopathological changes were observed. Results: The serum levels of ALT and AST in the model group increased. Hepatic lobules had necrotic foci and inflammatory cell infiltration. The plasma levels of TNF-α and IFN-γ increased. In liver tissues, the levels of NP, MDA and MPO rose, but that of SOD decreased. Magnesium isoglycyrrhizinate significantly attenuated the activities of ALT and AST (P<0.05). Histopathological staining showed that inflammation of the liver was relieved significantly. Magnesium isoglycyrrhizinate also decreased the levels of NP, MDA and MPO in liver tissues (P<0.05), raised that of SOD and reduced the plasma levels of TNF-α and IFN-γ (P<0.05). Conclusion: Magnesium isoglycyrrhizinate protected against ConA-induced immunological liver injury in mice, probably through immune regulation and antioxidation.

Keywords

1. Zhou YX, Mo SL, Wang RH, Liu WF, Liu JC, Wang JQ, et al. Corn baked by burning coal triggered overexpression of osteopontin in hepatocytes of rats following fluorosis. Toxicol Ind Health. 2012;28(3):195-202. DOI: 10.1177/074823371141090510.1177/0748233711410905Open DOISearch in Google Scholar

2. Wang J, Zhao P, Guo H, Sun X, Jiang Z, Xu L, et al. Serum IL-33 levels are associated with liver damage in patients with chronic hepatitis C. Mediators Inflamm. 2012;2012:819636. DOI: 10.1155/2012/819636Search in Google Scholar

3. Ingawale DK, Mandlik SK, Naik SR. Models of hepatotoxicity and the underlying cellular, biochemical and immunological mechanism(s): a critical discussion. Environ Toxicol Pharmacol. 2014;37(1):118-33. DOI: 10.1016/j.etap.2013.08.01510.1016/j.etap.2013.08.015Open DOISearch in Google Scholar

4. Rijckborst V, Sonneveld MJ, Janssen HL. Review article: chronic hepatitis B - anti-viral or immunomodula-tory therapy? Aliment Pharmacol Ther. 2011;33(5):501-13. DOI: 10.1111/j.1365-2036.2010.04555.x10.1111/j.1365-2036.2010.04555.xOpen DOISearch in Google Scholar

5. de Groen RA, Boltjes A, Hou J, Liu BS, McPhee F, Friborg J, et al. IFN-λ-mediated IL-12 production in macrophages induces IFN-γ production in human NK cells. Eur J Immunol. 2015;45(1):250-9. DOI: 10.1002/eji.20144490310.1002/eji.201444903Open DOISearch in Google Scholar

6. Yang Q, Zhao X, Zang L, Fang X, Zhao J, Yang X, et al. Anti-hepatitis B virus activities of α-DDB-FNC, a novel nucleoside-biphenyldicarboxylate compound in cells and ducks, and its anti-immunological liver injury effect in mice. Antiviral Res. 2012;96(3):333-9. DOI: 10.1016/j.antiviral.2012.10.00310.1016/j.antiviral.2012.10.003Open DOISearch in Google Scholar

7. Erhardt A, Biburger M, Papadopoulos T, Tiegs G. IL-10, regulatory T cells, and Kupffer cells mediate tolerance in concanavalin A-induced liver injury in mice. Hepatology. 2007;45(2):475-85. DOI: 10.1002/hep.21498Search in Google Scholar

8. Xie C, Li X, Wu J, Liang Z, Deng F, Xie W, et al. Anti-inflammatory Activity of Magnesium Isoglycyrrhizinate Through Inhibition of Phospholipase A2/Arachidonic Acid Pathway. Inflammation. 2015;38(4):1639-48. DOI: 10.1007/s10753-015-0140-2Search in Google Scholar

9. Tiegs G, Hentschel J, Wendel A. AT cell-dependent experimental liver injury in mice inducible by concanavalin A. J Clin Invest. 1992;90(1):196-203. DOI: 10.1172/JCI11583610.1172/JCI115836Open DOISearch in Google Scholar

10. Gantner F, Leist M, Jilg S, Germann PG, Freudenberg MA, Tiegs G. Tumor necrosis factor-induced hepatic DNA fragmentation as an early marker of T cell-dependent liver injury in mice. Gastroenterology. 1995;109(1):166-76. DOI: 10.1016/0016-5085(95)90282-1Search in Google Scholar

11. Yu JJ, Zhang CS, Coyle ME, Du Y, Zhang AL, Guo X, et al. Compound glycyrrhizin plus conventional therapy for psoriasis vulgaris: a systematic review and meta-analysis of randomized controlled trials. Curr Med Res Opin. 2017;33(2):279-87. DOI: 10.1080/03007995.2016.125460510.1080/03007995.2016.1254605Open DOISearch in Google Scholar

12. van Rossum TGJ. In: Glycyrrhizin treatment for Chronic Hepatitis C. Erasmus University Rotterdam. 2000; retrieved from http://hdl.handle.net/1765/20441.Search in Google Scholar

13. Wang HX, Liu M, Weng SY, Li JJ, Xie C, He HL, et al. Immune mechanisms of Concanavalin A model of autoimmune hepatitis. World J Gastroenterol. 2012;18(2):119-25. DOI: 10.3748/wjg.v18.i2.11910.3748/wjg.v18.i2.119Open DOISearch in Google Scholar

14. Li WW, Yu JY, Xu HL, Bao JK. Concanavalin A: a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics. Biochem Biophys Res Commun. 2011;414(2):282-6. DOI: 10.1016/j.bbrc.2011.09.07210.1016/j.bbrc.2011.09.072Open DOISearch in Google Scholar

15. Jakubowski A, Sternak M, Jablonski K, Ciszek-Lenda M, Marcinkiewicz J, Chlopicki S. 1-Methylnicotin-amide protects against liver injury induced by concanavalin A via a prostacyclin-dependent mechanism: A possible involvement of IL-4 and TNF-α. Int Immunopharmacol. 2016;31:98-104. DOI: 10.1016/j.intimp.2015.11.03210.1016/j.intimp.2015.11.032Open DOISearch in Google Scholar

16. Li J, Gong YM, Wu J, Wu WJ, Cai W. Anti-tumor necrosis factor-α monoclonal antibody alleviates parenteral nutrition-associated liver disease in mice. JPEN J Parenter Enteral Nutr. 2012;36(2):219-25. DOI: 10.1177/014860711142441210.1177/0148607111424412Open DOISearch in Google Scholar

17. Nicoletti F, Zaccone P, Xiang M, Magro G, Di Mauro M, Di Marco R, et al. Essential pathogenetic role for interferon (IFN-)gamma in concanavalin A-induced T cell-dependent hepatitis: exacerbation by exogenous IFN-gamma and prevention by IFN-gamma receptor-immunoglobulin fusion protein. Cytokine. 2000;12(4):315-23. DOI: 10.1006/cyto.1999.056110.1006/cyto.1999.0561Open DOISearch in Google Scholar

18. Capuron L, Geisler S, Kurz K, Leblhuber F, Sperner-Unterweger B, Fuchs D. Activated immune system and inflammation in healthy ageing: relevance for tryptophan and neopterin metabolism. Curr Pharm Des. 2014;20(38):6048-57. DOI: 10.2174/138161282066614031711021710.2174/1381612820666140317110217Open DOISearch in Google Scholar

19. Ceccarelli G, Brenchley JM, Cavallari EN, Scheri GC, Fratino M, Pinacchio C, et al. Impact of High-Dose Multi-Strain Probiotic Supplementation on Neurocognitive Performance and Central Nervous System Immune Activation of HIV-1 Infected Individuals. Nutrients. 2017;9(11):E1269. DOI: 10.3390/nu911126910.3390/nu9111269Open DOISearch in Google Scholar

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