rss_2.0Nova Biotechnologica et Chimica FeedSciendo RSS Feed for Nova Biotechnologica et Chimicahttps://sciendo.com/journal/NBEChttps://www.sciendo.comNova Biotechnologica et Chimica 's Coverhttps://sciendo-parsed-data-feed.s3.eu-central-1.amazonaws.com/6005b40de797941b18f244c8/cover-image.jpg?X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20210727T030244Z&X-Amz-SignedHeaders=host&X-Amz-Expires=604800&X-Amz-Credential=AKIA6AP2G7AKDOZOEZ7H%2F20210727%2Feu-central-1%2Fs3%2Faws4_request&X-Amz-Signature=b4f2552bc6a48540256e03885ec61a5107fdf7a0df712cce58ad8d4545b4d0a8200300Bioinformatic Analysis of Prophage Endolysins and Endolysin-Like Genes from the Order Lactobacillaleshttps://sciendo.com/article/10.2478/v10296-012-0001-4<abstract xml:lang="en"><title style='display:none'>Bioinformatic Analysis of Prophage Endolysins and Endolysin-Like Genes from the Order Lactobacillales</title><p>Endolysins belonging to the group of peptigoglycan hydrolases, which are able to cleave peptidoglycan in bacterial cell walls, become an extensively studied group of enzymes. Thanks to their narrow target specificity and low probability of resistance they are considered to be an appropriate alternative to conventional antibiotics. The present paper concerns the occurrence of endolysin and endolysin-like genes in genomes of bacteria belonging to the order Lactobacillales. Using bioinformatic programmes we compared and analysed protein sequences of catalytic and cell wall binding (CWB) domains of these enzymes, their preferred combinations, their phylogenetic relationship and potential occurence of natural "domain shuffling". The existence of this phenomenon in selected group of enzymes was confirmed only in limited range, so we assume that the natural trend is the distribution of "well-tried" combinations of catalytic and CWB domains of endolysin genes as a whole.</p></abstract>ARTICLE2012-06-28T00:00:00.000+00:00Synthesis, Complex Compounds and Antimicrobial Activity of Some Derivatives of Furo[3,2-]Pyridine and Their Starting Compoundshttps://sciendo.com/article/10.2478/v10296-012-0008-x<abstract xml:lang="en"><title style='display:none'>Synthesis, Complex Compounds and Antimicrobial Activity of Some Derivatives of Furo[3,2-<italic>C</italic>]Pyridine and Their Starting Compounds</title><p>Some [3,2<italic>-c</italic>]pyridine derivatives were synthesized. 3-(Furan-2-yl)propenoic acid (<bold>1a</bold>) was prepared from furan-2-carbaldehyde under the Perkin's conditions. Obtained acid was converted to the corresponding azide <bold>3</bold>, which in turn was cyclized to give furo[3,2-<italic>c</italic>]pyridin-4(5<italic>H</italic>)-one (<bold>4a</bold>). The reaction of pyridone <bold>4a</bold> with phosphorus oxychloride rendered the chloroderivative <bold>7a</bold>, which was treated in the condition of Suzuki coupling reaction with boronic acid to give 4-phenylfuro[3,2-<italic>c</italic>]pyridine (<bold>8e</bold>) and an unexpected product <bold>10</bold>. Some title compounds have shown moderate to good antimicrobial activity against tested bacteria <italic>Xanthomonas sp., Erwinia amylovora</italic>, and filamentous fungi <italic>Pyrenophora avenae, Fusarium graminearum.</italic></p></abstract>ARTICLE2012-06-28T00:00:00.000+00:00Antioxidant Activity of Milling Fractions of Selected Cerealshttps://sciendo.com/article/10.2478/v10296-012-0005-0<abstract xml:lang="en"><title style='display:none'>Antioxidant Activity of Milling Fractions of Selected Cereals</title><p>The aim of this study was to evaluate antioxidant potential of four milling fractions of selected cereals grew in the year 2009 and 2010. Free radical scavenging activity of samples was measured using DPPH assay and reducing power was determined using FRAP assay. Secondary, total phenolic and flavonoid content of cereal extracts was evaluated. We found that flour fractions (break flour and reduction flour) showed the lower proportion of the total antioxidant potential than bran fractions (fine bran and coarse bran), which was balanced in observed years. Extract from barley had the highest values of antioxidant activity and polyphenol content.</p></abstract>ARTICLE2012-06-28T00:00:00.000+00:00Chemometrics in Medicine and Pharmacyhttps://sciendo.com/article/10.2478/v10296-012-0002-3<abstract xml:lang="en"><title style='display:none'>Chemometrics in Medicine and Pharmacy</title><p>This minireview summarizes the basic ways of application of chemometrics in medicine and pharmacy. It brings a collection of applications of chemometric used for the solution of diverse practical problems, e.g. exploitation of biologically active species, effective use of biomarkers, advancement of clinical diagnosis, monitoring of the patient's state and prediction of its perspectives, drug design or classification of toxic chemical substances. The aim of this contribution is a brief presentation of versatile potentialities of contemporary chemometrical techniques and relevant software. They are exemplified by typical cases from literature as well as by own research results of the Chemometrics group at Department of Chemistry, the University of Ss. Cyril &amp; Methodius in Trnava.</p></abstract>ARTICLE2012-06-28T00:00:00.000+00:00Solid-Phase Extraction for Photometric Determination of Rosmarinic Acid in Lemon Balm () Extractshttps://sciendo.com/article/10.2478/v10296-012-0007-y<abstract xml:lang="en"><title style='display:none'>Solid-Phase Extraction for Photometric Determination of Rosmarinic Acid in Lemon Balm (<italic>Melissa Officinalis</italic>) Extracts</title><p>The aim of this study was evaluation of the solid-phase extraction for elimination of interference compounds from lemon balm extracts aimed for photometric determination of rosmarinic acid. In experiments, evaluated conditions were as follows: composition and volume of mobile phase, ratio between volume of sample and mass of stationary phase and flow rate of mobile phase during separation. The results indicated that interfered compounds were eliminated. The lemon balm extracts should be pretreated by adsorption on normal stationary phase (silica gel) in ratio sample volume to silica gel weight 1:1 (v/w) elution by mobile phase - diethyl ether: acetic acid (9:1; v/v) - volume - 40 times of crude extract volume - with flow rate 5 ml/min. After selection of SPE conditions, the method was validated with comparison to HPLC analysis. The results suggest that this method may be useable for determination of rosmarinic acid by photometric measurement based on the complexation of Fe<sup>2+</sup> ions with rosmarinic acid.</p></abstract>ARTICLE2012-06-28T00:00:00.000+00:00Optimization of Barley Mature Embryo Regeneration and Comparison with Immature Embryos of Local Cultivarshttps://sciendo.com/article/10.2478/v10296-012-0006-z<abstract xml:lang="en"><title style='display:none'>Optimization of Barley Mature Embryo Regeneration and Comparison with Immature Embryos of Local Cultivars</title><p>Regeneration ability of plant cells or tissues in explant culture is one of the key factors affecting success of genetic transformation. In experiments, the effect of explant type (whole embryo, scutellum embryonic axis, meristematic/central zone of embryonic axis) and plant growth regulators (BAP or TDZ) on mature embryo regeneration was determined. Explant type significantly affected regeneration efficiency. While no regenerants were observed using mature scutella, whole embryos or embryonic axes produced the highest number of regenerants. Using embryonic axes with discarded apical and basal parts, regeneration efficiency dramatically decreased. No statistical differences in regeneration were observed between BAP and TDZ added to the regeneration medium in concentration 0.1 or 1 mg l<sup>-1</sup>. At last, regeneration ability of mature embryos of nine Slovak spring barley cultivars (Donaris, Ezer, Levan, Ludan, Nitran, Pribina Sladar, Orbit, Pax) and Golden Promise as a model cultivar was examined and compared with regeneration ability of immature embryos which have been usually used for genetic transformation of barley. Although the regeneration from mature embryos was very weak, the same cultivars Golden Promise, Pribina and Levan showed the best regeneration ability by using both, immature and mature embryos. On the other hand cultivars Ezer and Pax belonged to the weakest ones in both experiments.</p></abstract>ARTICLE2012-06-28T00:00:00.000+00:00Monitoring of Polycyclic Aromatic Hydrocarbons (Pahs) in Surface Water of the Hungarian Upper Section of the Danube Riverhttps://sciendo.com/article/10.2478/v10296-012-0003-2<abstract xml:lang="en"><title style='display:none'>Monitoring of Polycyclic Aromatic Hydrocarbons (Pahs) in Surface Water of the Hungarian Upper Section of the Danube River</title><p>The aim of this paper is to investigate the concentrations of polycyclic aromatic hydrocarbons (PAHs) in surface water of the Hungarian upper section of the Danube River in the period of 2007-2010. A total of 77 water samples were collected from the sampling sites located at Rajka, Medve and Komárom (1848, 1806 and 1766 river km) under the authority of the Inspectorate for Environment, Nature and Water of the North Transdanubian Region designated by the Hungarian National Monitoring Programme. Sixteen PAHs identified by the US Environmental Protection Agency (USEPA) as priority pollutants were monitored.</p><p>Concentrations of total 16 PAHs (ΣPAHs) ranged from 25 to 357 ng·L<sup>-1</sup> with the mean value of 98.27 ± 58.48 ng·L<sup>-1</sup>. The low and medium molecular weight PAHs (2-3 and 4 ring) ranged from below method detection limit (&lt;1) to 136 ng·L<sup>-1</sup> while high molecular weight PAHs (5-6 ring) were present at much lower concentrations (&lt;1-25 ng·L<sup>-1</sup>). The 2-3-ring PAHs contributed to about 64% while 4-6-ring PAHs accounted for 36% of the ΣPAHs. The dominant species are naphthalene and phenanthrene in the surface water. Concentration ratios of specific PAH compounds including anthracene/(anthracene+phenanthrene) and fluoranthene/(fluoranthene+pyrene) were calculated to evaluate the possible sources of PAH contamination. The levels of ΣPAHs determined in our study were compared with other sections of the Danube and other regions of the world.</p></abstract>ARTICLE2012-06-28T00:00:00.000+00:00Quantitative Structure-Antioxidant Activity Relationship of Quercetin and its New Synthetised Derivativeshttps://sciendo.com/article/10.2478/v10296-012-0004-1<abstract xml:lang="en"><title style='display:none'>Quantitative Structure-Antioxidant Activity Relationship of Quercetin and its New Synthetised Derivatives</title><p>Interest in the biological activity of the flavonoids increases due to the potential health benefits of these polyphenolic components of foodstuff. Our research investigates biological properties of the flavonoids and their new synthetized derivatives, focuses on the relationship between their antioxidant activity and their chemical structures.</p><p>Quantitative structure-activity relationship (QSAR) attempts to correlate chemical structure with biological activity using statistical approaches. It is the process by which chemical structure of a molecule is quantitatively correlated with a well defined process, such as biological activity, that can be expressed quantitatively as the concentration of a substance required to give a certain biological response. When physicochemical properties or structures are expressed by numbers, the mathematical relation can be formed between the two. The mathematical expression can then be used to predict the biological response of other chemical compounds.</p><p>QSARs represent predictive models derived from application of statistical tools correlating antioxidant activity (including desirable therapeutic effect and undesirable side effects) of chemicals with descriptors representative of molecular structure and properties. Obtaining a good QSAR model depends on many factors, such as the quality of biological data, the choice of descriptors and statistical methods. Any QSAR modeling should ultimately lead to statistically robust models capable of making accurate and reliable predictions of biological activities of new untested compounds.</p></abstract>ARTICLE2012-06-28T00:00:00.000+00:00Metagenomic Analysis of Slovak Bryndza Cheese Using Next-Generation 16S rDNA Amplicon Sequencinghttps://sciendo.com/article/10.1515/nbec-2016-0003<p> Knowledge about diversity and taxonomic structure of the microbial population present in traditional fermented foods plays a key role in starter culture selection, safety improvement and quality enhancement of the end product. Aim of this study was to investigate microbial consortia composition in Slovak bryndza cheese. For this purpose, we used culture-independent approach based on 16S rDNA amplicon sequencing using next generation sequencing platform. Results obtained by the analysis of three commercial (produced on industrial scale in winter season) and one traditional (artisanal, most valued, produced in May) Slovak bryndza cheese sample were compared. A diverse prokaryotic microflora composed mostly of the genera Lactococcus, Streptococcus, Lactobacillus, and Enterococcus was identified. Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris were the dominant taxons in all tested samples. Second most abundant species, detected in all bryndza cheeses, were Lactococcus fujiensis and Lactococcus taiwanensis, independently by two different approaches, using different reference 16S rRNA genes databases (Greengenes and NCBI respectively). They have been detected in bryndza cheese samples in substantial amount for the first time. The narrowest microbial diversity was observed in a sample made with a starter culture from pasteurised milk. Metagenomic analysis by high-throughput sequencing using 16S rRNA genes seems to be a powerful tool for studying the structure of the microbial population in cheeses.</p>ARTICLE2016-08-04T00:00:00.000+00:00Electrochemical Treatment of Water Contaminated with Methylorangehttps://sciendo.com/article/10.1515/nbec-2016-0006<p>This study examines electrochemical degradation of water artificially contaminated by azo dye Methyl Orange (MO). Degradation is based on chemical electro-oxidation of MO molecules. Graphite was used as an electrode material for electrochemical oxidation of MO. In this work, the different operative parameters (electric current, NaCl content) and their effect on effectiveness as well as the treatment time/duration of MO degradation were tested. The highest dye removal (91.0 %) was obtained during the electrolysis at current density 3.032 mA/cm<sup>2</sup>, electrolyte with the content of NaCl 4 g/dm<sup>3</sup> (NaCl) and the treatment time 35 min.</p>ARTICLE2016-08-04T00:00:00.000+00:00Improved Multiplex Polymerase Chain Reaction for Rapid Staphylococcus Aureus Detection in Meat and Milk Matriceshttps://sciendo.com/article/10.1515/nbec-2016-0007<p> Staphylococcal food poisoning represents one of the most frequently occurring intoxications, caused by staphylococcal enterotoxins (SE-s) and staphylococcal enterotoxin-like proteins (SEl-s). Therefore, there is a need for rapid, sensitive and specific detection method for this human pathogen and its toxin genes in food matrices. The present work is focused on Staphylococcus aureus detection by a nonaplex polymerase chain reaction, which targets the 23S rRNA gene for identification of S. aureus at the species level, genes for classical SE-s (SEA, SEC, SED), new SE-s (SEH, SEI), SEl-s (SEK, SEL) and tsst-1 gene (toxic shock syndrome toxin). Primers were properly designed to avoid undesirable interactions and to create a reliably identifiable profile of amplicons when visualized in agarose gel. According to obtained results, this approach is able to reach the detection sensitivity of 12 colony forming units from milk and meat matrices without prior culturing and DNA extraction.</p>ARTICLE2016-08-04T00:00:00.000+00:00Degradation of Synthetic Dyes by Laccases – A Mini-Reviewhttps://sciendo.com/article/10.1515/nbec-2016-0010<p> Laccases provide a promising future as a tool to be used in the field of biodegradation of synthetic dyes with different chemical structures. These enzymes are able to oxidize a wide range of phenolic substrates without the presence of additional co-factors. Laccases have been confirmed for their potential of synthetic dye degradation from wastewater and degradation products of these enzymatic reactions become less toxic than selected dyes. This study discusses the potential of laccase enzymes as agents for laccase-catalyzed degradation in terms of biodegradation efficiency of synthetic dyes, specifically: azo dyes, triphenylmethane, indigo and anthraquinone dyes. Review also summarizes the laccase-catalyzed degradation mechanisms of the selected synthetic dyes, as well as the degradation products and the toxicity of the dyes and their degradation products.</p>ARTICLE2016-08-04T00:00:00.000+00:00In Vitro Regeneration Potential of Seven Commercial Soybean Cultivars (Glycine max L.) for Use in Biotechnologyhttps://sciendo.com/article/10.1515/nbec-2016-0001<p> This work is aimed to evaluate in vitro regeneration potential of seven commercial soybean varieties Bohemians, Cardiff, Gallec, Merlin, Moravians, Naya and Silensia (Glycine max L.) cultivated in Central Europe. Our results showed the half-seeds could be effectively used as an explant source for all tested cultivars. The regeneration was initiated on the media containing growth regulators 1.67 mg.l-1 BAP and 0.25 mg.l<sup>-1</sup> GA<sub>3</sub>. Within the first five days culture, green chlorophyll-containing explants were observed with frequency from 18.3% to 55.9%. Two weeks later, the explants responded by production of calli with the efficiency up to 83.0%. First shoots appeared after 2–3 weeks of subculture on the media. The soybean regeneration showed to be genotype-dependent with variable efficiencies from 5.7% (cv. Naya) to 37.7% (cv. Gallec). The cultivars Cardiff, Merlin and Gallec appear to be the most promising candidates for further biotechnological use. Application of antioxidants such as L-cysteine, dithiothreitol and sodium thiosulfate does not have effect on the explant regeneration for the first five days.</p>ARTICLE2016-08-04T00:00:00.000+00:00Preliminary Results on Growing Second Generation Biofuel Crop Miscanthus X Giganteus at The Polluted Military Site in Ukrainehttps://sciendo.com/article/10.1515/nbec-2016-0008<p> The semi-field research on using second-generation biofuel crop Miscanthus x giganteus for restoration of former military site in Kamenetz-Podilsky, Ukraine was carried out during two vegetation seasons. Despite high metal pollution of soil, in particular, by Fe, Mn, Ti, and Zr, no growth inhibition was observed. The concentrations followed pattern soil &gt; roots &gt; stems &gt; leaves. Accumulation of particular metals in roots was different: Fe, Mn and Ti were accumulated rather palpably after the first vegetation season and less tangible after the second one. Cu, Pb and Zn were less accumulative in both vegetation seasons, and for As and Pb the accumulative concentrations were very small. Accumulations in the aboveground parts of the plant in comparison to roots were significantly lower in case of Fe, Ti, Mn, Cu, Zn, Sr and even statistically comparable to zero in case of As, Pb and Zr. Calculated translocation ratio of metals in the plant’s parts preferably indicated lack of metals’ hyper accumulation. Generally, no correlations were observed between concentrations of metals in the soil and in the upper plant’s parts. The research confirmed the ability of Miscanthus x giganteus to grow on the military soils predominantly contaminated by metals.</p>ARTICLE2016-08-04T00:00:00.000+00:00Targeting transgene to seed resulted in high rate of morphological abnormalities of transformantshttps://sciendo.com/article/10.2478/nbec-2019-0012<p>False flax (<italic>Camelina sativa</italic> L.) is currently under-exploited but highly promising oilseed crop. Combining <italic>Camelina’s</italic> attractive agronomic traits with its unprecedented ease for genetic engineering makes it an ideal plant chassis for biotechnology applications, in particular synthetic biology strategies. For targeted expression of transgene particularly to seeds requires identification and application of seed specific promoters. In the present study two cultivars of <italic>Camelina,</italic> namely Zuzana and Smilowska, were used for transformation at early flowering stage using the floral dip method. The plants were inoculated with <italic>Agrobacterium</italic> bearing a construct for expression of red fluorescent protein (RFP) under the control of the seed specific cruciferin promoter <italic>CRUC</italic> from <italic>Arabidopsis</italic>. Transgenic seeds and plants were identified on the basis of red fluorescence (RFP) and kanamycin resistance. Relatively high transformation efficiency of 8 % was achieved particularly for the cultivar Zuzana. However, many of regenerants exerted developmental deformations such as lack of shoot apical meristem, deformed or absent cotyledons, etc. Furthermore, the activity of the <italic>CRUC</italic> promoter was still active also in true leaves rendering this promoter as inappropriate for seed targeting of the transgene. Nevertheless, genetic transformation remains a tool for direct modulation of pathways for oil synthesis in oilseed crops.</p>ARTICLE2019-12-26T00:00:00.000+00:00Deleterious amino acid substitutions with a series of putative damaging effects on egg components are revealed in the ovalbumin gene family; an approachhttps://sciendo.com/article/10.2478/nbec-2019-0014<p>This study was conducted to identify the most deleterious nonsynonymous single nucleotide polymorphisms (nsSNPs) in the ovalbumin gene family, including <italic>OVALX</italic>, <italic>OVALY,</italic> and <italic>OVAL</italic> genes, which are involved in the synthesis of the most important components in the chickens’ eggs using a comprehensive <italic>in silico</italic> approach. Ten different computational servers were utilized to prioritize the possible deleterious effects of the retrieved nsSNPs in terms of structure, function, and stability. Results indicated entirely damaging effects of <italic>H365P</italic> in <italic>OVALX</italic>, <italic>I167T</italic> in <italic>OVALY</italic>, and <italic>V209G</italic>, <italic>L231P</italic>, <italic>F307C</italic>, and <italic>S317P</italic> in <italic>OVAL</italic> proteins. Further prediction tools showed that all of these deleterious nsSNPs were positioned in variable locations within several α-helix motifs in all studied ovalbumin proteins. Furthermore, all witnessed nsSNPs were predicted to be resided in the receptors binding sites, signifying remarkable involvement of such nsSNPs in damaging of the altered proteins. In conclusion, the present study provides the first inclusive data with regard to the most deleterious nsSNPs in <italic>OVALX</italic>, <italic>OVALY</italic> and <italic>OVAL</italic> genes in chickens. The present bioinformatics data may be useful for breeders who intend to raise chickens for egg production, in such a way the presence of any of these deleterious nsSNPs in any selected breed may possess several damaging effects on the egg components, which may impair egg production. Therefore, it can be stated that breeders have to confirm the absence of any of these deleterious nsSNPs before being proceeded further for large-scale egg-production purposes.</p>ARTICLE2019-12-26T00:00:00.000+00:00Identification of and potential metabolites of novel cardiovascular and adrenolytic drugs by liquid chromatography-mass spectrometry with the aid of experimental designhttps://sciendo.com/article/10.2478/nbec-2019-0020<p>Drug metabolism in liver microsomes was studied <italic>in vitro</italic> using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Relevant drug was incubated with dog, human and rat liver microsomes (DLMs, HLMs, RLMs) along with NADPH, and the reaction mixture was analyzed by LC-MS/MS to obtain specific metabolic profile. GRACE analytical C18 column, Vision HT (50 × 2 mm, 1.5 μm) was implemented with acetonitrile and water (+ 5 mM ammonium acetate) in a gradient mode as the mobile phase at a flow 0.4 mL.min<sup>−1</sup>. Different phase I and phase II metabolites were detected and structurally described. The metabolism of the studied drugs occurred <italic>via</italic> oxidation, hydroxylation and oxidative deamination processes. Conjugates with the glucuronic acid and sulfate were also observed as phase II biotransformation. The central composite design (CCD) showed that factors, such as time incubation, liver microsomal enzymes concentration and NADPH concentration, along with drying gas temperature, nebulizer gas pressure and capillary voltage significantly affected the final response of the method. This study describes the novel information about the chemical structure of the potential metabolites of selected biologically active compounds, which provide vital data for further pharmacokinetic and <italic>in vivo</italic> metabolism studies.</p>ARTICLE2019-12-26T00:00:00.000+00:00Behavior of metallic trace elements containing in stabilized and solidified oily petroleum sludgehttps://sciendo.com/article/10.2478/nbec-2019-0018<p>The present work examines the influence of the leaching conditions on the release of various chemical elements from a cementitious material obtained by solidification of an industrial waste rejection of Algeria. Toxicity Characteristic Leaching Procedure (TCLP), X-ray diffraction (XRD) and Scanning electron microscopy coupled with energy dispersive X-ray microanalysis SEM-EDX analyses were employed to characterize the waste and the stabilized/solidified materials. Than several formulations were prepared with different percent of waste ranging from 0 % to 30 %. To evaluate the influence of leaching conditions on the release of chemical ions (Zn<sup>2+</sup>, Pb<sup>2+</sup>, Cl-, Mg<sup>2+</sup>, Ca<sup>2+</sup>, Na<sup>+</sup>, K<sup>+</sup> and SO<sub>4</sub><sup>2−</sup>) contained in the stabilized and solidified materials, the Acid Neutralization Capacity (ANC), the Pore Water (PW) and Monolith Leaching Tests (MLT) have been carried out. The leaching tests (ANC, PW and MLT) have shown a low metal leachability. However, the lowest released amount was observed for the MLT.</p>ARTICLE2019-12-26T00:00:00.000+00:00The efficiency of a low-cost hydrogen sulphide (HS) kit as an early warning test for assessing microbial rainwater quality and its correlation with standard indicators microorganismshttps://sciendo.com/article/10.2478/nbec-2019-0016<p>Testing microbial quality of the harvested rainwater remains a challenge in many countries. The H<sub>2</sub>S test kit is a low-cost microbiological field-based test which can be used in areas where water testing facilities are limited. This study compares its efficiency with the standard indicators microorganisms in the detection of faecal contamination of rainwater in South Africa. A total of 88 rainwater samples were collected from various tanks in the Eastern Cape, South Africa over three months in 2016. The collected samples were analysed for faecal bacterial contamination using the H<sub>2</sub>S test kit, Colilert-18/Quanti-tray<sup>®</sup>/2000 and the membrane filtration technique for faecal coliforms (MFT). The correspondence rate of the H<sub>2</sub>S test kit with MFT was 88 %, while for the Colilert<sup>®</sup> it was 76 %. The H<sub>2</sub>S test kit confirmed faecal contamination when concentrations of standards indicators microorganisms were 5 most-probable number of cells/100 cm<sup>3</sup> or higher. Overall, the best correspondence of the H<sub>2</sub>S test kit with Colilert<sup>®</sup> was observed at <italic>E. coli</italic> concentrations above 50 most-probable number of cells/100 cm<sup>3</sup>. Results of the H<sub>2</sub>S test kit correlated better with MTF, while the medium used has strongly influenced the enumeration of faecal contamination. Results point to strong effect of media used and revealed the need to calibrate the correspondence between the standard indicator microorganisms and the H<sub>2</sub>S test kit under local conditions for specific settings.</p>ARTICLE2019-12-26T00:00:00.000+00:00Radiocesium Adsorption By Zeolitic Materials Synthesized From Coal Fly Ashhttps://sciendo.com/article/10.2478/nbec-2014-0007<p> Brown coal fly ash derived from the combustion of brown-coal in power plant ENO Nováky (Slovak Republic) was used as raw material for synthesis of zeolitic materials ZM1 and ZM3 by hydrothermal alternation with 1M NaOH and 3M NaOH, respectively. Fly ash and synthesized products were characterized using XRF and SEM-EDX analysis. Subsequently, zeolitic materials were tested as sorbents to remove Cs<sup>+</sup> ions from aqueous solutions using radiotracer technique. Sorption of cesium by both types of zeolitic materials obeys Langmuir adsorption isotherm model. The maximum sorption capacities Q<sub>max</sub> at pH 6.0 calculated from Langmuir isotherm were 1203 ± 65 μmol Cs<sup>+</sup>/ g for ZM1 and 1341 ± 66 μmol Cs<sup>+</sup>/ g for ZM3. The results showed that alkali treated fly ash can be used as effective sorbent for radiocesium removal from contaminated solutions</p>ARTICLE2014-07-10T00:00:00.000+00:00en-us-1