1. bookVolume 19 (2019): Edizione 3 (July 2019)
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eISSN
2300-8733
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25 Nov 2011
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access type Accesso libero

The Effect of Different Concentrations of Caffeine, Pentoxifylline and 2’-Deoxyadenosine on the Biological Properties of Frozen-Thawed Canine Semen

Pubblicato online: 30 Jul 2019
Volume & Edizione: Volume 19 (2019) - Edizione 3 (July 2019)
Pagine: 733 - 746
Ricevuto: 06 Jan 2019
Accettato: 14 Mar 2019
Dettagli della rivista
License
Formato
Rivista
eISSN
2300-8733
Prima pubblicazione
25 Nov 2011
Frequenza di pubblicazione
4 volte all'anno
Lingue
Inglese
Abstract

Artificial insemination (AI) and semen cryopreservation are the most accessible and commonly used techniques for breeding domestic animals. Among many parameters, such as plasma membrane integrity and acrosome structure, one of the key factors that determine the quality of frozen-thawed samples for artificial insemination is sperm motility. Sperm motility is one of the key parameters that determine the quality of frozen-thawed samples for AI. The total number of progressively motile spermatozoa in thawed canine semen is correlated with fertility. A variety of substances were used to compare sperm motility with the control. The aim of this study was to determine the effect of semen extender supplementation with motility stimulants, pentoxifylline (PTX), caffeine (CAF) and 2’-deoxyadenosine (DX), after different post-thaw incubation times (30, 60, 120 min) on the motility, selected kinematic parameters, plasma membrane integrity and mitochondrial membrane potential of cryopreserved canine spermatozoa. During attempts to improve the quality of cryopreserved semen, the applied substances exerted beneficial effects at a concentration of 10 mM. We demonstrated that both phosphodiesterase inhibitors, caffeine and pentoxifylline, as well as 2’-deoxyadenosine increased the motility and selected kinematic parameters of thawed canine spermatozoa.

Keywords

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