Anti-inflammatory and antioxidative effects of genistein in a model of spinal cord injury in rats
, , , , , , y | 29 oct 2021
Article Category: Original article
Publicado en línea: 29 oct 2021
Páginas: 233 - 243
DOI: https://doi.org/10.2478/abm-2021-0029
Palabras clave
© 2021 Ercan Bal et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
Neurological damage from spinal cord injury (SCI) is a result of primary mechanical injury and secondary damage from oxidative stress and neuroinflammation. Although genistein has been shown to have potent antioxidant and anti-inflammatory effects in studies of brain injury, its effect on secondary damage in SCI has remained unknown.
Objective
To determine effects of genistein in a model of SCI in rats.
Methods
We divided 21 rats evenly into 3 groups, a control group, in which only a laminectomy was performed; a trauma group in which SCI was induced; and a genistein group in which genistein was administered subcutaneously after SCI. The rats were assessed using a Basso–Beattie and Bresnahan functional score at the 12th hour and on the 1st, 3rd, 5th, and 7th days. Biochemical analyses were conducted at the same time points to determine the serum levels of catalase, ischemia-modified albumin (IMA), disulfide (SS), total thiol (TT), native thiol (NT), disulfide/total thiol (SS/TT), and native thiol/total thiol (NT/TT). Total oxidant and antioxidant capacity, and oxidative stress index were determined in spinal cord tissue obtained on the 7th day together with immunohistochemistry for cyclooxygenase-2 levels.
Result
Catalase activity on the 7th day was significantly (
Conclusion
Genistein has antioxidant, anti-inflammatory, and protective effects in a model of SCI in rats and warrants further study.