<abstract xmlns="http://www.w3.org/1999/xhtml"><bold>Introduction:</bold> Orf virus (ORFV) is a prototype <italic>Parapoxvirus</italic> species in the Poxviridae family that causes serious zoonotic infectious disease. Goat skin fibroblast (GSF) cells are the major host targets of ORFV. Interleukin 8 (IL-8) and tumour necrosis factor (TNF)-α are known to play a vital role in immune response during viral infections. However, the manner of variation over time of their level of expression in GSF cells remains unclear.<bold>Material and Methods:</bold> In this study, quantitative enzyme-linked immunosorbent assay chips were used to detect changes in the levels of these cytokines expressed and secreted in GSF cells after ORFV infection.<bold>Results:</bold> Results showed that the expression of IL-8, TNF-α, and decorin was upregulated in the cell lysates, and that secreted decorin and IL-8 were significantly increased in cell supernatant.<bold>Conclusion:</bold> The results provided possible approaches to elucidation of how ORFV infection initiates host cell immune response.</abstract>

Introduction: Orf virus (ORFV) is a prototype Parapoxvirus species in the Poxviridae family that causes serious zoonotic infectious disease. Goat skin fibroblast (GSF) cells are the major host targets of ORFV. Interleukin 8 (IL-8) and tumour necrosis factor (TNF)-α are known to play a vital role in immune response during viral infections. However, the manner of variation over time of their level of expression in GSF cells remains unclear.Material and Methods: In this study, quantitative enzyme-linked immunosorbent assay chips were used to detect changes in the levels of these cytokines expressed and secreted in GSF cells after ORFV infection.Results: Results showed that the expression of IL-8, TNF-α, and decorin was upregulated in the cell lysates, and that secreted decorin and IL-8 were significantly increased in cell supernatant.Conclusion: The results provided possible approaches to elucidation of how ORFV infection initiates host cell immune response.

<italic>Parapoxvirus</italic> orf virus infection induces an increase in interleukin-8, tumour necrosis factor-α, and decorin in goat skin fibroblast cells

State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou, 730046,

Journal of Veterinary Research

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

 orf virus infection induces an increase in interleukin-8, tumour necrosis factor-α, and decorin in goat skin fibroblast cells

Introduction: Orf virus (ORFV) is a prototype Parapoxvirus species in the Poxviridae family that causes serious zoonotic infectious disease. Goat skin...

Parapoxvirus orf virus infection induces an increase in interleukin-8, tumour necrosis factor-α, and decorin in goat skin fibroblast cells

Published Online: Sep 23, 2016

Page range: 239 - 243

Received: Feb 23, 2016

Accepted: Sep 05, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0036

Keywords
goat skin fibroblasts, orf virus, cytokines

© 2016 Lingling Wang et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Parapoxvirus orf virus infection induces an increase in interleukin-8, tumour necrosis factor-α, and decorin in goat skin fibroblast cells

Published Online: Sep 23, 2016

Page range: 239 - 243

Received: Feb 23, 2016

Accepted: Sep 05, 2016

DOI: https://doi.org/10.1515/jvetres-2016-0036

Keywordsgoat skin fibroblasts, orf virus, cytokines

© 2016 Lingling Wang et al., published by De Gruyter Open

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
goat skin fibroblasts, orf virus, cytokines