Room-temperature stable loop-mediated isothermal amplification (LAMP) reagents to detect leptospiral DNA
, , and | Aug 20, 2021
Article Category: Technical report
Published Online: Aug 20, 2021
Page range: 183 - 189
DOI: https://doi.org/10.2478/abm-2021-0023
Keywords
© 2021 Pui-Yuei Lee et al., published by Sciendo
This work is licensed under the Creative Commons Attribution 4.0 International License.
Background
Loop-mediated isothermal amplification (LAMP) is one of the most promising tools for rapidly detecting
Objective
To overcome the drawback of cold storage requirement for LAMP reagents we modified the reagents by adding sucrose as stabilizer. We then sought to determine the stability at room temperature of the premixed LAMP reagents containing sucrose.
Method
Premixed LAMP reagents with sucrose and without sucrose were prepared. The prepared mixtures were stored at room temperature for up to 60 days, and were subjected to LAMP reactions at various intervals using rat kidney samples to detect leptospiral DNA.
Results
The premixed LAMP reagents with sucrose remained stable for 45 days while sucrose-free premixed LAMP reagents showed no amplification from day 1 of storage at room temperature up to day 14.
Conclusion
The LAMP reagent system can be refined by using sucrose as stabilizer, thus allowing their storage at room temperature without the need for cold storage. The modified method enables greater feasibility of LAMP for field surveillance and epidemiology in resource-limited settings.