<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>Pesticide use has increased steadily over the years in both industrial farming and local agriculture. One of the most widely used pesticides at a global level, glyphosate, has been controversial for many years and many studies have focused on the effects of this compound on human health. Ethephon is also a controversial ripening agent used to ripen crops more quickly. A basic but crucial step in the study of the effects of their use on human health is the development of adequate analytical methodologies for the quantification of the compounds in relevant samples. For this study a quick, simple and selective method which uses LC-MS/MS was developed for the determination of ethephon, glyphosate and AMPA. The method uses selective fragment monitoring for each analyte and the internal standard, without prior analytical separation. Mobile phase used consisted of aqueous ammonium formate and methanol in isocratic elution and the sample cleanup was made using solid phase extraction (SPE). The method was validated with regards to selectivity, sensitivity, accuracy and precision in accordance with applicable guidelines. After validation the LC-MS/MS method was successfully used to determine ethephon, glyphosate and AMPA residues in ground and surface water, as well as vegetable samples.</p>
</abstract>

Pesticide use has increased steadily over the years in both industrial farming and local agriculture. One of the most widely used pesticides at a global level, glyphosate, has been controversial for many years and many studies have focused on the effects of this compound on human health. Ethephon is also a controversial ripening agent used to ripen crops more quickly. A basic but crucial step in the study of the effects of their use on human health is the development of adequate analytical methodologies for the quantification of the compounds in relevant samples. For this study a quick, simple and selective method which uses LC-MS/MS was developed for the determination of ethephon, glyphosate and AMPA. The method uses selective fragment monitoring for each analyte and the internal standard, without prior analytical separation. Mobile phase used consisted of aqueous ammonium formate and methanol in isocratic elution and the sample cleanup was made using solid phase extraction (SPE). The method was validated with regards to selectivity, sensitivity, accuracy and precision in accordance with applicable guidelines. After validation the LC-MS/MS method was successfully used to determine ethephon, glyphosate and AMPA residues in ground and surface water, as well as vegetable samples.

A Quick, Simple, Sensitive and Selective LC-MS/MS Method Used for the Screening of Ethephon, Glyphosate and Aminomethylphosphonic Acid from Water and Food Samples

George Emil Palade University of Medicine, Pharmacy, Science, and Technology

Department of Occupational Medicine, Faculty of Medicine

Department of Health Protection, National Health Laboratory (LNS)

Acta Biologica Marisiensis

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Pesticide use has increased steadily over the years in both industrial farming and local agriculture. One of the most widely used pesticides at a global level,...

A Quick, Simple, Sensitive and Selective LC-MS/MS Method Used for the Screening of Ethephon, Glyphosate and Aminomethylphosphonic Acid from Water and Food Samples

Published Online: Jul 09, 2022

Page range: 41 - 54

Received: May 27, 2022

Accepted: Jun 14, 2022

DOI: https://doi.org/10.2478/abmj-2022-0005

Keywords
glyphosate, AMPA, ethephon, LC-MS, QTOF, biomonitoring, pesticide

© 2022 Lénárd Farczádi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

A Quick, Simple, Sensitive and Selective LC-MS/MS Method Used for the Screening of Ethephon, Glyphosate and Aminomethylphosphonic Acid from Water and Food Samples

Published Online: Jul 09, 2022

Page range: 41 - 54

Received: May 27, 2022

Accepted: Jun 14, 2022

DOI: https://doi.org/10.2478/abmj-2022-0005

Keywordsglyphosate, AMPA, ethephon, LC-MS, QTOF, biomonitoring, pesticide

© 2022 Lénárd Farczádi et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Keywords
glyphosate, AMPA, ethephon, LC-MS, QTOF, biomonitoring, pesticide