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Comparison of RT-qPCR results of different gene targets for SARS-CoV-2 in asymptomatic individuals during COVID-19 pandemic

Gulten Tuncel
Gulten Tuncel
, 
Mahmut Cerkez Ergoren
Mahmut Cerkez Ergoren
, 
Buket Baddal
Buket Baddal
, 
Pinar Tulay
Pinar Tulay
, 
Ayse Arikan
Ayse Arikan
, 
Emrah Guler
Emrah Guler
, 
Cenk Serhan Ozverel
Cenk Serhan Ozverel
, 
H. Kaya Suer
H. Kaya Suer
, 
Murat Sayan
Murat Sayan
, 
Tamer Sanlidag
Tamer Sanlidag
 and 
   | Jul 24, 2021
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Article Category: COVID-19 Special Issue
Published Online: Jul 24, 2021
Page range: 26 - 31
DOI: https://doi.org/10.2478/ebtj-2021-0018
Keywords
© 2021 Gulten Tuncel, Mahmut Cerkez Ergoren, Buket Baddal, Pinar Tulay, Ayse Arikan, Emrah Guler, Cenk Serhan Ozverel, H. Kaya Suer, Murat Sayan, Tamer Sanlidag, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Figure 1A

Diagnovital SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control).
Diagnovital SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control).

Figure 1B

Bio-Speedy SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control)
Bio-Speedy SARS-CoV-2 RT-qPCR amplification results in Insta Q96TM Plus Real-time PCR Detection System (PC: positive control, NTC: no template control)

Figure 2

Comparison of detection rate and Ct values of Diagnovital® kit detecting N1 and N2 genes and Bio-Speedy® kit detecting RdRp gene. Graph depicts Ct values obtained for all samples (n=43) in all RT-PCR assays. The lines show the mean Ct value for each assay, triangles show the Ct values of the samples with the highest (sample 28) and lowest (sample 22) value. The detection rate for each kit is indicated below the data points, e.g. 10/43 means 10 out of 43 samples tested positive according to the instructions for data interpretation provided by the manufacturer
Comparison of detection rate and Ct values of Diagnovital® kit detecting N1 and N2 genes and Bio-Speedy® kit detecting RdRp gene. Graph depicts Ct values obtained for all samples (n=43) in all RT-PCR assays. The lines show the mean Ct value for each assay, triangles show the Ct values of the samples with the highest (sample 28) and lowest (sample 22) value. The detection rate for each kit is indicated below the data points, e.g. 10/43 means 10 out of 43 samples tested positive according to the instructions for data interpretation provided by the manufacturer

The comparison of two different targeted COVID-19 RT-qPCR kits in positive specimens.

Sample No RT-qPCR Cycle Thhreshold Value (Ct)
Diagnovital® N1+N2 Bio-Speedy® RdRp
1 32.98 Negative
2 32 Negative
3 30.22 Negative
4 38.96 Negative
5 20.33 35.33
6 30.60 Negative
7 33.36 33.71
8 36.36 Negative
9 34.01 Negative
10 27.93 33.89
1 22.02 27.17
12 37.93 Negative
13 30.71 Negative
14 29.88 34.19
15 28.41 30.20
16 38.41 Negative
17 29.34 Negative
18 31.44 Negative
19 36.24 Negative
20 35.81 Negative
21 36.80 Negative
2 21.55 28.19
23 29.95 Negative
24 36.68 Negative
25 37.64 Negative
26 33.39 Negative
27 28.73 Negative
28 40.00 Negative
29 37.07 Negative
30 32.42 Negative
31 37.03 Negative
32 32.21 Negative
3 33.10 Negative
34 25.54 34.73
35 22.25 31.15
36 28.29 Negative
37 33.05 Negative
38 39.94 Negative
39 32.75 Negative
40 26.21 35.21
41 34.45 Negative
42 28.18 Negative
43 37.27 Negative
PC 1 28.60 27.57
PC 2 28.28 26.20
Total 43 10
eISSN:
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Language:
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Life Sciences, Genetics, Biotechnology, Bioinformatics, other
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