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Application of a liposomal subunit vaccine in chickens for reduction of Campylobacter gut colonisation

Anna Łasica

Anna Łasica

Department of Bacterial Genetics, Institute of MicrobiologyWarszawa, Poland
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Łasica, Anna
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Renata Godlewska

Renata Godlewska

Department of Bacterial Genetics, Institute of MicrobiologyWarszawa, Poland
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Godlewska, Renata
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Jerzy Gubernator

Jerzy Gubernator

Department of Lipids and Liposomes, Faculty of Biotechnology, University of WroclawWrocław, Poland
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Gubernator, Jerzy
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Anna Jakubiak-Augustyn

Anna Jakubiak-Augustyn

Department of Lipids and Liposomes, Faculty of Biotechnology, University of WroclawWrocław, Poland
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Jakubiak-Augustyn, Anna
, 
Paweł Majewski

Paweł Majewski

Department of Animal Physiology, Institute of Functional Biology and Ecology, University of WarsawWarszawa, Poland
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Majewski, Paweł
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Agnieszka Wyszyńska

Agnieszka Wyszyńska

Department of Bacterial Genetics, Institute of MicrobiologyWarszawa, Poland
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Wyszyńska, Agnieszka
  
Nov 06, 2024
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Published Online: Nov 06, 2024
Page range: 487 - 496
Received: Jun 02, 2024
Accepted: Oct 28, 2024
DOI: https://doi.org/10.2478/jvetres-2024-0062
Keywords
© 2024 Anna Łasica et al., published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Introduction

Campylobacter are the most common cause of food poisoning, which manifests itself in diarrhoea of varying severity. Additionally, because of the increasing number of people with immune deficiencies, more frequent serious complications of Campylobacter infections are being observed. The main source of infection is the consumption of contaminated poultry meat, which is a consequence of the insufficiency of current hygiene and biosecurity to control Campylobacter or eliminate it from the poultry food chain.

Material and Methods

Two hybrid proteins, presenting selected epitopes of the Campylobacter antigens CjaD and EF-Tu, were developed based on the highly immunogenic proteins CjaA and CjaC. Four groups of chickens were vaccinated with different preparations (a mixture of both hybrid proteins encapsulated in anionic or neutral liposomes) and different doses (a single dose given on the day of hatching or two doses given on days 1 and 14 of life). The number of Campylobacter was assessed in the intestinal contents of vaccinated birds.

Results

No statistically significant differences in colonisation levels were observed between chickens immunised with neutral liposomes containing hybrid proteins and their non-immunised counterparts, regardless of dosage regimen.

Conclusion

Although immunisation of chickens did not produce the expected results, the approach used has great potential, which is worth further investigation and development.
Language:
English
Publication timeframe:
4 times per year
Journal Subjects:
Life Sciences, Molecular Biology, Microbiology and Virology, Life Sciences, other, Medicine, Veterinary Medicine
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