<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The root of <italic>Paris polyphylla</italic> var. <italic>yunnanensis</italic>, a famous and endangered traditional Chinese herb, has a significant medicinal value. The aim of this study was to analyze the composition and functional characteristics of bacterial endophytes in roots, stems, and leaves of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic>. The 16S rRNA gene sequencing and functional prediction of bacterial endophytes in roots, stems, and leaves of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> were conducted. The Chao and Shannon indices of the bacteria in roots were significantly higher than those in stems and leaves. The dominant endophyte phyla were Cyanobacteria, Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The main genera detected in roots were unclassified <italic>Cyanobacteria, Rhizobium, Flavobacterium</italic>, and <italic>Sphingobium</italic>; the main genera in stems were <italic>norank_c__Cyanobacteria, Bacillus</italic>, and <italic>Pseudomonas</italic>; the main genera in leaves were <italic>norank_c__Cyanobacteria</italic> and <italic>Rhizobium</italic>. The microbiota in roots was particularly enriched in functional categories “extracellular structures” and “cytoskeleton” compared with stems and leaves (<italic>p</italic> &lt; 0.05). Our study reveals the structural and functional characteristics of the endophytic bacteria in roots, stems, and leaves of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic>, which aids in the scientific understanding of this plant.</p>
</abstract>

The root of Paris polyphylla var. yunnanensis, a famous and endangered traditional Chinese herb, has a significant medicinal value. The aim of this study was to analyze the composition and functional characteristics of bacterial endophytes in roots, stems, and leaves of P. polyphylla var. yunnanensis. The 16S rRNA gene sequencing and functional prediction of bacterial endophytes in roots, stems, and leaves of P. polyphylla var. yunnanensis were conducted. The Chao and Shannon indices of the bacteria in roots were significantly higher than those in stems and leaves. The dominant endophyte phyla were Cyanobacteria, Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The main genera detected in roots were unclassified Cyanobacteria, Rhizobium, Flavobacterium, and Sphingobium; the main genera in stems were norank_c__Cyanobacteria, Bacillus, and Pseudomonas; the main genera in leaves were norank_c__Cyanobacteria and Rhizobium. The microbiota in roots was particularly enriched in functional categories “extracellular structures” and “cytoskeleton” compared with stems and leaves (p < 0.05). Our study reveals the structural and functional characteristics of the endophytic bacteria in roots, stems, and leaves of P. polyphylla var. yunnanensis, which aids in the scientific understanding of this plant.

Bacterial Diversity in Roots, Stems, and Leaves of Chinese Medicinal Plant <italic>Paris polyphylla</italic> var. <italic>yunnanensis</italic>


Polish Journal of Microbiology

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Bacterial Diversity in Roots, Stems, and Leaves of Chinese Medicinal Plant  var. 


The root of Paris polyphylla var. yunnanensis, a famous and endangered traditional Chinese herb, has a significant medicinal value. The aim of this study was...

Index	Mean ± SD (R)	Mean ± SD (S)	Mean ± SD (L)	p-value (R-S)	p-value (L-S)	p-value (L-R)
Shannon	2.66 ± 0.72	1.55 ± 0.58	0.72 ± 0.28	1.51E-02	1.07E-02	1.10E-04
Chao1	614.36 ± 44.24	467.46 ± 111.12	386.30 ± 66.16	1.32E-02	1.55E-01	3.63E-05

Bacterial Diversity in Roots, Stems, and Leaves of Chinese Medicinal Plant Paris polyphylla var. yunnanensis

Article Category: original-paper

Published Online: Mar 11, 2020

Page range: 91 - 97

Received: Oct 09, 2019

Accepted: Feb 13, 2020

DOI: https://doi.org/10.33073/pjm-2020-012

Keywords
var., roots, stems, leaves, endophytic microbiota

© 2020 Tian-Hao Liu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

Fig. 8.

Microbial diversity indices in roots, stems, and leaves (mean ± SD) (n = 6).

Bacterial Diversity in Roots, Stems, and Leaves of Chinese Medicinal Plant Paris polyphylla var. yunnanensis

Article Category: original-paper

Published Online: Mar 11, 2020

Page range: 91 - 97

Received: Oct 09, 2019

Accepted: Feb 13, 2020

DOI: https://doi.org/10.33073/pjm-2020-012

Keywordsvar., roots, stems, leaves, endophytic microbiota

© 2020 Tian-Hao Liu et al., published by Sciendo

This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Fig. 5.

Fig. 6.

Fig. 7.

Fig. 8.

Microbial diversity indices in roots, stems, and leaves (mean ± SD) (n = 6).

Keywords
var., roots, stems, leaves, endophytic microbiota