<abstract xmlns="http://www.w3.org/1999/xhtml">

<p>The present study has investigated the effect of oil rich medium supplementation with polysorbates Tween 20, 40 and 80, for the cultivation of red oleaginous yeast <italic>Rhodotorula glutinis</italic>. <italic>R. glutinis</italic> has been cultivated in yeast extract peptone glucose modified broth (mYPG) supplemented with 2 % of waste cooking rapeseed oil and three polysorbate types with 0.5 %, 1 %, 2%, 3 %, 4 %, 5 %, 6 %, 7 %, 8 %, 9 % and 10 % concentration each. Yeast biomass was measured by the thermogravimetric method at 105 °C each day during 7-day experiment. The oil rich medium supplementation with Tween 20, 40 and 80 at concentrations ranging from 2 % to 10 % significantly increased the biomass of <italic>R. glutinis</italic>. All three types of the studied polysorbates with 0.5 % and 1 % concentration, did not affect yeast growth and the dry biomass – results were similar to the control sample without polysorbate addition. Between the three types of polysorbates, Tween 20 was selected as the preferable for <italic>R. glutinis</italic> cultivation with an optimal concentration of 2 %. Cultivation of <italic>R. glutinis</italic> in oil rich medium with polysorbates Tween 20, 40 and 80, supplementation up to 10 % concentration did not have had an inhibitory effect on the biomass growth.</p>
</abstract>

The present study has investigated the effect of oil rich medium supplementation with polysorbates Tween 20, 40 and 80, for the cultivation of red oleaginous yeast Rhodotorula glutinis. R. glutinis has been cultivated in yeast extract peptone glucose modified broth (mYPG) supplemented with 2 % of waste cooking rapeseed oil and three polysorbate types with 0.5 %, 1 %, 2%, 3 %, 4 %, 5 %, 6 %, 7 %, 8 %, 9 % and 10 % concentration each. Yeast biomass was measured by the thermogravimetric method at 105 °C each day during 7-day experiment. The oil rich medium supplementation with Tween 20, 40 and 80 at concentrations ranging from 2 % to 10 % significantly increased the biomass of R. glutinis. All three types of the studied polysorbates with 0.5 % and 1 % concentration, did not affect yeast growth and the dry biomass – results were similar to the control sample without polysorbate addition. Between the three types of polysorbates, Tween 20 was selected as the preferable for R. glutinis cultivation with an optimal concentration of 2 %. Cultivation of R. glutinis in oil rich medium with polysorbates Tween 20, 40 and 80, supplementation up to 10 % concentration did not have had an inhibitory effect on the biomass growth.

Effect of Polysorbates on the Growth of <italic>Rhodotorula Glutinis</italic> in Oil Rich Medium

Latvia University of Life Sciences and Technologies

Environmental and Climate Technologies

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of Polysorbates on the Growth of  in Oil Rich Medium

The present study has investigated the effect of oil rich medium supplementation with polysorbates Tween 20, 40 and 80, for the cultivation of red oleaginous...

Effect of Polysorbates on the Growth of Rhodotorula Glutinis in Oil Rich Medium

Published Online: Dec 04, 2021

Page range: 1075 - 1085

DOI: https://doi.org/10.2478/rtuect-2021-0081

Keywords
Dry biomass, oil emulsification, oleaginous yeast, Tween, waste cooking oil

© 2021 Svetlana Raita et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Effect of Polysorbates on the Growth of Rhodotorula Glutinis in Oil Rich Medium

Published Online: Dec 04, 2021

Page range: 1075 - 1085

DOI: https://doi.org/10.2478/rtuect-2021-0081

KeywordsDry biomass, oil emulsification, oleaginous yeast, Tween, waste cooking oil

© 2021 Svetlana Raita et al., published by Sciendo

This work is licensed under the Creative Commons Attribution 4.0 International License.

Keywords
Dry biomass, oil emulsification, oleaginous yeast, Tween, waste cooking oil