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Fig. 1.

Electrophoresis pattern of Pestalotiopsis kenyana PG52 genome. Agarose concentration (%): 1; voltage: 180 V; time: 35 min.; molecular weight standard name: M1: λ-Hind Ш digest (Takara), M2: D2000 (Tiangen); sample volume: M1: 3 μl, M2: 6 μl.
Electrophoresis pattern of Pestalotiopsis kenyana PG52 genome. Agarose concentration (%): 1; voltage: 180 V; time: 35 min.; molecular weight standard name: M1: λ-Hind Ш digest (Takara), M2: D2000 (Tiangen); sample volume: M1: 3 μl, M2: 6 μl.

Fig. 2.

Pestalotiopsis kenyana PG52 base composition distribution map. The X axis represents the position on reads, and the Y axis represents the percentage of bases.
Pestalotiopsis kenyana PG52 base composition distribution map. The X axis represents the position on reads, and the Y axis represents the percentage of bases.

Fig. 3.

Pestalotiopsis kenyana PG52 base mass distribution map. The X axis is the position of the base in reads, and the Y axis is the base quality value. Each point in the figure represents the total number of bases at this position that reach a certain.
Pestalotiopsis kenyana PG52 base mass distribution map. The X axis is the position of the base in reads, and the Y axis is the base quality value. Each point in the figure represents the total number of bases at this position that reach a certain.

Fig. 4.

KEGG function analysis.
KEGG function analysis.

Fig. 5.

GO functional classification map of all unigenes with GO annotation.
GO functional classification map of all unigenes with GO annotation.

Fig. 6.

COG function analysis.
COG function analysis.

Glycosyl hydrolase families related to mycoparasitic in mycoparasites.

SpeciesGH18GH75GH17GH55GH64GH81
Pestalotiopsis sp. PG52  3671932
Trichoderma harzianum2044  532
Trichoderma atroviride2955  832
Trichoderma virens36541031

The number of polyketide synthases and nonribosomal peptide synthetases of P. fici, Pestalotiopsis sp. NC0098 and mycoparasites.

Secondary metabolitesPestalotiopsis sp. PG52P. ficiPestalotiopsis sp. NC0098T. harzianumT. atrovirideT. virens
NRPS  131212171628
PKS1022721271818
Total1153933443446

The comparison of Pestalotiopsis genome sequences.

PG52FICINC0098
Assembly size (Mb)555246.61
Scaffold N50 (Mb)  6.6  4.0  5
Coverage (fold)335.024.524
GC content (%)  53.3048.7351.28
Protein-coding genes20,02315,41315,180
Gene density (genes per Mb)345.22296.90327.08
Exons per gene    3.13    2.76    2.83

The genome is compared with the transcription group.

GenesTranscription groupsGenomeExpression rate
PKS  8210280.39%
NRPS  10  1376.92%
Protease13717578.29%
Cytochrome P45024531777.29%
Zn2Cys6 transcription factor    1    425.00%

Numbers of P450, protease and Zn2/Cys6 transcription factor genes of mycoparasites.

Pestalotiopsis sp. PG52T. harzianumT. atrovirideT. virens
Cytochrome P450317501540
Zn2/Cys6 transcription factor   4  76995
Protease175532328
eISSN:
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Language:
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Journal Subjects:
Life Sciences, Microbiology and Virology